| Bt基因导入马铃薯品种大西洋的研究 |
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| 引用本文： | 苏俊峰,卢翠华,田兴亚,李晶,于凤丽,邸宏,高丽娜.Bt基因导入马铃薯品种大西洋的研究[J].中国马铃薯,2004,18(5):257-260. |
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| 作者姓名： | 苏俊峰 卢翠华 田兴亚 李晶 于凤丽 邸宏 高丽娜 |
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| 作者单位： | 东北农业大学,哈尔滨,150030 |
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| 摘 要： | 通过基因工程手段,利用农杆菌介导法,将Bt基因导入马铃薯品种大西洋,同时对马铃薯品种大西洋的叶片再生体系进行优化,探讨了影响植株再生频率和遗传转化效率的因素,确立了最佳植株再生体系和遗传转化条件,获得了转基因植株。
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| 关 键 词： | 马铃薯 再生 遗传转化 Bt基因 |
| 文章编号： | 1672-3635(2004)05-0257-04 |
| 修稿时间： | 2004年7月30日 |
INTRODUCTION OF BT GENE INTO THE POTATO CULTIVAR ATLANTIC |
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| SU Jun-feng,LU Cui-hua,TIAN Xing-ya,LI Jing,YU Feng-li,DI Hong,GAO Li-na.INTRODUCTION OF BT GENE INTO THE POTATO CULTIVAR ATLANTIC[J].Chinese Potato,2004,18(5):257-260. |
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| Authors: | SU Jun-feng LU Cui-hua TIAN Xing-ya LI Jing YU Feng-li DI Hong GAO Li-na |
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| Abstract: | In this study, Bt gene was introduced into cv. Atlantic successfully with Agrobacterium-mediated method. At the same time, the regeneration system of the cultivar Atlantic was optimized for leaf explants. We discussde the factors which effect the regeneration rate and the transformation rate. A high frequency of regeneration system in vitro and genetic transformation system of potato were established, and transgenic plants were gained. |
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| Keywords: | potato regeneration genetic transformation Bt gene |
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