| 马铃薯SSR标记聚丙烯酰胺凝胶电泳分析 |
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| 引用本文： | 王绍鹏,邱彩玲,李勇,宿飞飞,刘尚武,吕典秋.马铃薯SSR标记聚丙烯酰胺凝胶电泳分析[J].中国马铃薯,2009,23(3):143-147. |
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| 作者姓名： | 王绍鹏 邱彩玲 李勇 宿飞飞 刘尚武 吕典秋 |
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| 作者单位： | 黑龙江省农业科学院植物脱毒苗木研究所,黑龙江省马铃薯工程技术研究中心,黑龙江哈尔滨150086 |
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| 基金项目： | 黑龙江省科技厅国际合作项目资助 |
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| 摘 要： | 以马铃薯品种早大白、黄麻子、克新13、荷兰15、大西洋为试验材料,提取基因组DNA,进行SSR标记,并将标记产物进行聚丙烯酰胺凝胶电泳,比较不同电泳参数(聚丙烯酰胺凝胶的浓度、电泳电压、染色方法)对电泳结果的影响。确定了适合马铃薯SSR标记的电泳检测体系,即聚丙烯酰胺凝胶的浓度为12%,电泳电压为170V,染色方法为溴化乙锭染色。
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| 关 键 词： | 马铃薯 SSR标记 聚丙烯酰胺凝胶 溴化乙锭 |
Analysis of Potato with Polyacrylamide Gel Electrophoresis Based on SSR Markers |
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| Wang Shaopeng,Qiu Cailing,Li Yong,Su Feifei,Liu Shangwu,Lu Dianqiu.Analysis of Potato with Polyacrylamide Gel Electrophoresis Based on SSR Markers[J].Chinese Potato,2009,23(3):143-147. |
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| Authors: | Wang Shaopeng Qiu Cailing Li Yong Su Feifei Liu Shangwu Lu Dianqiu |
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| Abstract: | The genomic DNA was extracted from five potato varieties, Zaodabai, Huangmazi, Kexin 13, Favorita and Atlantic.The effects were compared of the electrophoresis parameters of gel concentration, voltage and stain substrate on the outcomes of electrophoresis by polyacrylamide gel.The optimal electrophoresis conditions for potato SSR markers were 12% gel concentration, 170 V voltage and EB as staining reagent. |
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| Keywords: | potato SSR markers polyacrylamide gel ethidium bromide staining |
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