Creating a novel herbicide-tolerance OsALS allele using CRISPR/Cas9-mediated gene editing |
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作者姓名: | Fangquan Wang Yang Xu Wenqi Li Zhihui Chen Jun Wang Fangjun Fan Yajun Tao Yanjie Jiang Qian-Hao Zhu Jie Yang |
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作者单位: | Institute of Food Crops;Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops;Provincial Key Laboratory of Agrobiology;CSIRO Agriculture and Food;Institute of Life Science |
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基金项目: | This study was supported by the National Transgenic Science and Technology Program(2018ZX08001-02B);the Jiangsu Agricultural Science and Technology Innovation Fund(CX(19)3059);the Jiangsu Province Key Research and Development Program(Modern Agriculture,BE2017345-2). |
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摘 要: | Weeds and weedy rice plague commercial rice fields in many countries. Developingherbicide-tolerance rice is the most efficient strategy to control weed proliferation. CRISPR/Cas9-mediated gene editing, which generates small InDels and nucleotide substitutions atand around target sites using error-prone non-homologous end joining DNA repairing, hasbeen widely adopted for generation of novel crop germplasm with a wide range of geneticvariation in important agronomic traits. We created a novel herbicide-tolerance allele inrice by targeting the acetolactate synthase (OsALS) gene using CRISPR/Cas9-mediated geneediting. The novel allele (G628W) arose from a G-to-T transversion at position 1882 of OsALSand conferred a high level of herbicide tolerance. Transgene-free progeny carryinghomozygous G628W allele were identified and showed agronomic performance similar tothat of wild-type plants, suggesting that the G628W allele is a valuable resource fordeveloping elite rice varieties with strong herbicide tolerance. To promote use of the G628Wallele and to accelerate introgression and/or pyramiding of the G628W allele with other elitealleles, we developed a DNA marker for the G628W allele that accurately and robustlydistinguished homozygous from heterozygous segregants. Our result further demonstratesthe feasibility of CRISPR/Cas9-mediated gene editing in creating novel genetic variation forcrop breeding.
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关 键 词: | RICE CRISPR/Cas9 ALS Herbicide-tolerance Crop breeding |
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