利用RAPD技术鉴定花生种质资源的差异

利用RAPD技术对 7个不同植物学类型的花生种质资源进行了分析 ,在所用的 83个随机引物中 ,1 3个引物的扩增产物显示出不同品种间的多态性 ,能显示花生品种间DNA多态性的引物占1 5 .7%。这 1 3个引物共扩增出 1 2 1条DNA带 ,其中具多态性的片段 2 6条 ,占 2 1 .48%。根据本实验结果 ,利用RAPD技术鉴定花生种质资源的多态性是有效的

RAPD in Cultivated Groundnut (Arachis hypogaea L.)

Seven cultivated groundnut accessions four botanical types have been studied for polymorphism using random amplified polymorphic DNA(RAPD) assay. Of the 83 oligonucleotide primers used only 13 (15.7%) yielded polymorphic amplification products. The total number of bands from the 13 primers is 121, of which 26 (21.48%) are polymorphic. Although the variation at DNA level is relatively less comparing with the extensive variation for phenotypic and agronomic traits, our results demonstrate that RAPD approach can be employed to generate DNA marker in groundnut.