| 花生DNA快速简便提取方法的研究 |
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| 引用本文： | 陈由强,庄伟建.花生DNA快速简便提取方法的研究[J].花生学报,1999(3):1-9. |
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| 作者姓名： | 陈由强 庄伟建 |
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| 作者单位： | 福建师范大学生物工程学院，福建农业大学甘蔗研究所 |
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| 基金项目： | 福建省教委科学研究基金 |
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| 摘 要： | 在花生新鲜针叶和干叶ＤＮＡ提取过程中，应用ｐＨ值较低，无机盐浓度较高的提取缓冲液可沉淀蛋白质，其中加入２％的β－巯基乙醇可有效地防止次生代谢物质使ＤＮＡ变色。提取出的ＤＮＡ样品产率在９２．６～２１６．３１ｎｇ／ｍｇ．ｆｗ，ＤＮＡ质量和纯度较高，２６０ｎｍ／２８０ｎｍ光密度比值在１．８～１．９之间。所得ＤＮＡ可直接用于限制性内切酶酶切，并可用于随机引物ＰＣＲ扩增。该方法为花生分子生物学研究提供基础。
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| 关 键 词： | 花生 DNA提取 限制性内切酶酶切 RAPD |
A Simple and Modified Procedure to Isolate Total DNA from Leaves of Peanut (Arachis hypogaea) |
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| Chen Youqiang,Ye Bingying,Zhu Jinmao.A Simple and Modified Procedure to Isolate Total DNA from Leaves of Peanut (Arachis hypogaea)[J].Journal of Peanut Science,1999(3):1-9. |
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| Authors: | Chen Youqiang Ye Bingying Zhu Jinmao |
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| Abstract: | A low pH extraction medium with high salts, which avoids ionization and oxidation of phenolic compounds during tissue grinding and precipitation of large amounts of materials, were successfully used to extract total DNA from the leaf of peanut (Arachis hypogaea).The DNA yields, quality and purity were characterized. These extracted DNA could be used directly for RAPD analysis, which are useful as molecular genetic markers, and digested with restriction enzymes. A fast ,inexpensive and reliable procedure has been developed for detecting the genetic diversity ofArachis hypogaea . |
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| Keywords: | Arachis hypogaea Isolation of total DNA Digestion with restriction enzyme |
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