Enhanced resistance to bacterial infection byErwinia carotovora subsp.atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes |
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Authors: | Patricio Arce Mauricio Moreno Mónica Gutierrez Marlene Gebauer Paola Dell’Orto Hebert Torres Ivette Acuña Pauline Oliger Alejandro Venegas Xavier Jordana Julio Kalazich Loreto Holuigue |
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Institution: | 1. Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, P.O. Box 114-D, Santiago, Chile 3. Centro Regional de Investigación Remehue, Instituto Nacional de Investigaciones Agropecuarias (INIA), P.O. Box 24-O, Osorno, Chile 4. Facultad de Agronomía, Pontificia Universidad Católica de Chile, P.O. Box 306, Santiago, Chile
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Abstract: | Blackleg and soft rot diseases, caused by the bacteriumErwinia carotovora, are among the diseases that cause important losses in culture and storage of potato. In this paper, we introduced bacterial resistance into potato, via genes encoding for proteins with antibacterial activity. For this purpose, potato clones were transformed either with the gene encoding the acidic attacin protein fromHyalophora cecropia, or with the gene encoding the cecropin analog peptide SB37. These clones were evaluated for soft rot and blackleg resistance, after inoculation with the bacterial strainErwinia carotovora subsp.atroseptica T7. Results reported in this paper indicate that a considerable percentage of the potato clones (15–22%) showed increased resistance to bacterial infection, revealed by reduced severity of blackleg or soft rot symptoms. Expression of the transgenes was demonstrated in some of the clones by Northern blot analysis. This is the first report indicating that expression of the gene encoding for an attacin protein and for the cecropin SB-37 peptide in transgenic potato confers increased resistance to bacterial infection. |
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