| RFLP analysis in rice by using ECL direct nucleic acid labeling and detection system |
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| 作者姓名： | ZHANGShanhong LIUBin LUOLin ZHUXiaoyuan YANGQiyun WUShangzhong |
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| 作者单位： | [1]GuangdongKeyLabofCellEngineeringinRiceBreeding,RiveRvsInst [2]PlantProtectionInst,GuangdongAcadofAgriUniv,Guangdong510640,China |
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| 摘 要： | ECL (Enhanced Chemiluminescence) direct nu cleic acid labeling and detection syslem were used for RFLP analysis in rice. The system involved directly labeling DNA probe with the enzyme horseradish peroxidase (HRP), and the detection of non-radioactive signal based on chemiluminescence, that was the generation of light via enzyme (HRP)-catalyzed reactions. It was a simple, safe,
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| 关 键 词： | 水稻 RFLP分子标记 增强化学发光核酸标记 辣根过氧化物酶 DNA探针 磷32 |
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