| 杨梅花色苷分离纯化制备矢车菊素-3-葡萄糖苷的研究 |
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| 引用本文： | 赖剑峰,杨荣玲,林耀盛,刘学铭.杨梅花色苷分离纯化制备矢车菊素-3-葡萄糖苷的研究[J].热带作物学报,2013,34(7):1354-1358. |
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| 作者姓名： | 赖剑峰 杨荣玲 林耀盛 刘学铭 |
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| 作者单位： | 1. 广东省农业科学院蚕业与农产品加工研究所 广东省农产品加工重点实验室——省部共建国家重点实验室培育基地 广东广州 510610;江西农业大学生物科学与工程学院,江西南昌 330045
2. 广东省农业科学院蚕业与农产品加工研究所 广东省农产品加工重点实验室——省部共建国家重点实验室培育基地 广东广州 510610 |
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| 基金项目： | 广东省科技计划项目（No. 2009B020415003）。 |
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| 摘 要： | 以杨梅汁为原料,分别选用95％pH3.0的盐酸乙醇和含体积分数为0.5％ HC1的甲醇溶液作为大孔树脂和葡聚糖凝胶的洗脱液,分离制备矢车菊素-3-葡萄糖苷.通过HPLC检测和标准品对照,经大孔树脂和葡聚糖凝胶纯化后,其纯度分别为29.05％和90.35％.
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| 关 键 词： | 杨梅花色苷 分离纯化 矢车菊素-3-葡萄糖苷 |
Purification of Cyanidin-3-glucosid from Waxberry Anthocyanins |
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| LAI Jianfeng,YANG Rongling,LIN Yaosheng and LIU Xueming.Purification of Cyanidin-3-glucosid from Waxberry Anthocyanins[J].Chinese Journal of Tropical Crops,2013,34(7):1354-1358. |
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| Authors: | LAI Jianfeng YANG Rongling LIN Yaosheng and LIU Xueming |
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| Institution: | Food Science ang Technology College, Guangdong Ocean University;Dehong Tropical Agriculture Institute;Agriculture College, Guangdong Ocean University;1 The Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences / Cultivation Base of State Key Laboratory Constructed by Guangdong Province and Ministry of Science and Technolgy-Guangdong Key Lab of Agricultural;The Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences / Cultivation Base of State Key Laboratory Constructed by Guangdong Province and Ministry of Science and Technolgy-Guangdong Key Lab of Agricultural P;The Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences / Cultivation Base of State Key Laboratory Constructed by Guangdong Province and Ministry of Science and Technolgy-Guangdong Key Lab of Agricultural P;The Sericulture & Farm Produce Processing Research Institute, Guangdong Academy of Agricultural Sciences / Cultivation Base of State Key Laboratory Constructed by Guangdong Province and Ministry of Science and Technolgy-Guangdong Key Lab of Agricultural P |
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| Abstract: | Cyanidin-3-glucosid was separated and purified successfully from waxberry anthocyanins by macroporous resin and Sephadex LH20 with 95%(V/V)ethanol under pH3.0 and 0.5%(V/V)HCl methanol solution as the eluents, respectively. The contents of cyanidin-3-glucoside of the products which were purified by macroporous resin and Sephadex LH20 were 29.05% and 90.35%, respectively, compared with standard substance by HPLC. The product after purification is expected to replace imported standard for qualitative and quantitative analysis. |
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| Keywords: | Active dry yeast Stress culture Endogenous in culture yeast Bacteria Conditions optimization of accumulation |
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