| 番茄NAM基因的克隆与遗传转化 |
| |
| 引用本文： | 杨春文,唐宁,李正国.番茄NAM基因的克隆与遗传转化[J].热带作物学报,2012,33(8):1460-1465. |
| |
| 作者姓名： | 杨春文 唐宁 李正国 |
| |
| 作者单位： | 重庆大学生命科学学院 重庆市高校功能基因与调控新技术重点实验室 重庆400030 |
| |
| 基金项目： | 教育部博士点基金项目（No. 20110191110029）；国家自然科学基金项目（No. 30972002；No. 31071798）；2010中央高校个人项目（CDJXS10230017）。 |
| |
| 摘 要： | 为了研究番茄NAM基因的功能,利用RT-PCR技术从番茄cDNA中分离了NAM(GenBank登录号:FJ435163.1)基因,该基因ORF全长1 053 bp,共编码351个氨基酸,N端含有NAC结构域.定量RT-PCR分析结果表明,该基因在番茄的根、茎、叶、花和果实中均有较强表达.为了进一步研究番茄NAM基因的功能,构建了NAM基因的超表达载体pLP100-35S-NAM,通过农杆菌介导法转化番茄,并获得抗性植株.经表型分析,转基因番茄植株矮小,生长受到抑制,叶片形态异常,表明NAM基因影响番茄顶端分生组织(SAM)的形成和叶片形态的发生.
|
| 关 键 词： | 番茄 NAM 克隆 遗传转化 |
Cloning and Genetic Transformation of NAM Gene in Tomato |
| |
| YANG Chunwen,TANG Ning and LI Zhengguo.Cloning and Genetic Transformation of NAM Gene in Tomato[J].Chinese Journal of Tropical Crops,2012,33(8):1460-1465. |
| |
| Authors: | YANG Chunwen TANG Ning and LI Zhengguo |
| |
| Institution: | College of Life Sciences, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission;College of Life Sciences, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission;College of Life Sciences, Chongqing University, Key Lab of Functional Gene and New Regulation Technologies under Chongqing Municipal Education Commission |
| |
| Abstract: | To understand the function of NAM gene in tomato,the full length cDNA NAM gene was amplified by RT-PCR from tomato cDNA,which contains a 1 053 bp open reading frame(ORF)encoding 351 amino acid residues,and contains NAC domain in its N terminus.Quantitative PCR analysis showed that NAM expressed highly in all tissues including root,stem,leaf,flower and fruit.To further investigate the function of tomato NAM gene,NAM overexpression vector pLP-35S-NAM was constructed,and transformed into tomato plants by Agrobacterium-mediated method.The NAM overexpressing plants showed a dwarf phenotype,the size of these plants was smaller than those of wild-type plants,the plant growth was inhibited,and the leaves of transgenic tomato were highly lobed shaped.In conclusion,NAM affected the formation of shoot apical meristem(SAM)and leaf morphogenesis in tomato. |
| |
| Keywords: | Tomato NAM Clone Transformation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
