大苞鞘石斛组织培养快繁研究

为筛选出大苞鞘石斛组培快繁体系各阶段最适培养基配方,以大苞鞘石斛无菌苗茎段为外植体,通过正交试验研究不同因素对大苞鞘石斛植株再生的影响。结果表明:拟原球茎诱导最适培养基为1/2MS+2,4-D0.2 mg/L+蔗糖30 g/L,诱导出的拟原球茎直径1.5 mm时将其从茎段上剥离,并接入原培养基继续诱导15~20 d;拟原球茎增殖最适培养基为1/2MS+NAA 1 mg/L+蔗糖30 g/L+椰乳10%,继代周期25~30 d;拟原球茎分化最适培养基为1/2MS+NAA 0.5 mg/L+蔗糖30 g/L+椰乳10%,分化培养需40 d;将分化的无菌苗继代于花宝2号3 g/L+蛋白胨2 g/L+IBA 1 mg/L+蔗糖30 g/L+椰乳10%生根诱导效果最好,培养45 d;生根壮苗最佳培养基为花宝1号3 g/L+蛋白胨2 g/L+蔗糖30 g/L+活性炭1 g/L,培养50 d。

Tissue Culture and Plant Regeneration of Dendrobium wardianum

In order to study the effects of different factors on the tissue culture and plant regeneration of Dendrobium wardianum,three kinds of factors of three levels were used by an orthogonal design.The stem segments of D.wardianum were used as the explants for tissue culture.Results showed that the optimum PLBs induction medium was 1/2MS+2,4-D 0.2 mg/L+Sugar 30 g/L.When the shoot bud diameter reached to 1.5 mm,it must be detached and transferred to the same media for 15-20 days.The optimum medium for PLBs proliferation was 1/2MS+NAA 1 mg/L+Sugar 30 g/L+CW 10％ and the appropriate subculture cycle was about 25 to 30 days.The optimum PLBs differentiation medium was 1/2MS+NAA 0.5 mg/L+Sugar 30 g/L+CW 10％.The aseptic seedling which obtained from the former culture stage was cultured on the root induction media of Hyponex No.2 3 g/L+Peptone 2 g/L+IBA 1 mg/L+Sugar 30 g/L+CW 10％ for 45 days.The optimal medium of strong plantlets and rootage was Hyponex No.1 3 g/L+Peptone 2 g/L+Sugar 30 g/L+Activated carbon 1 g/L.