火龙果茎多糖的超高压提取及抗氧化活性研究

为研究火龙果茎多糖的超高压提取工艺及抗氧化活性。在单因素试验基础上,采用Box-Behnken设计和响应面分析方法,确定超高压提取的最优工艺,并从清除ABTS自由基和DPPH自由基能力方面来评价火龙果茎多糖的体外抗氧化能力。结果表明,火龙果茎多糖的超高压提取的最佳工艺条件为:液固比10∶1(m L∶g)、超高压压力300 MPa、超高压时间4 min。在此工艺条件下多糖得率为(2.83±0.02)%。火龙果茎对ABTS自由基和DPPH自由基具有清除作用,对ABTS自由基和DPPH自由基的清除率IC_(50)分别为浓度为4.3 mg/m L和5.5 mg/m L时。

Ultra High Pressure Extraction and Antioxidannt Activity of Polysaccharides from Pulp Pitaya Stem

To study the technology of ultra high pressure extraction and analysis its antioxidannt activity of polysaccharides from pulp pitaya stem, on the basis of single factor experiments, the optimal processing conditions were determined by means of response surface analysis and Box-Behnken design. The results showed that the optimum extraction conditions of the polysaccharides from pulp pitaya stem were as follows: liquid to solid ratio10:1 (mL/g), pressure 300 MPa and extraction time 4min. Under the conditions, the extraction yield of the polysaccharides from pulp pitaya stem reached ( 2.83 ±0.02)%. The polysaccharides from pulp pitaya stem had the ABTS and DPPH scavenging activity. The mass concentration of IC50 was 4.3 mg/mL and 5.5 mg/mL, respectively. This study could provide useful references for the development and exploitation of the polysaccharides from pulp pitaya stem.