红树林植物海莲不同部位提取物抗氧化活性研究

植物抗氧化研究对寻找高效率、低毒性天然抗氧化剂具有重要的指导意义。以在海南分布较广的红树林植物海莲进行抗氧化活性研究,以期对海莲开发利用的可能性提供理论依据。采用清除DPPH自由基和清除ABTS自由基对海莲不同部位———根、茎、叶、花的乙酸乙酯提取物进行抗氧化能力评价,采用比色法和福林酚法测定总黄酮和总酚含量。结果显示,海莲茎的提取物在4个部位中抗氧化活性最好,清除二者的IC50分别为(0.234±0.053)mg/m L和(0.196±0.080)mg/m L,海莲中酚类物质含量较大,4个部位中茎的总酚含量最多,含量为13.2 mg。说明海莲各部位均显示出了较好的抗氧化能力,具有较好的开发潜力。

Antioxidant Aactivity of Extracts from Different Parts of the Mangrove Plant Bruguiera sexangula

The search for effective,non-toxic natural antioxidants of plant origin has ever since increased.studying on the antioxidant activity of Bruguiera sexangula,a mangrove plant with a wide distribution in Hainan,providing a theoretical basis for the development and utilization of B.sexangula.2,2'-diphenyl-l-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radical scavenging activity were used to evaluate the antioxidant capacity of root,stem,leaf and flower parts of B.sexangula extracted by ethyl acetate,meanwhile,colorimetry and folin-ciocalteu were used to determine total flavonoids content (TFC) and total phenolic content (TPC).Results:stem part showed the most potent radical-scavenging activity on DPPH and ABTS radicals with IC50 value of (0.196±0.080)mg/mL and (0.234±0.053)mg/mL,respectively,and had a large content of phenols,13.2 mg.all parts of B.sexangula exhibited strong antioxidant activity and had good development potential.