金鱼草花青素糖基转移酶基因的克隆与表达特性分析

以白苏子(Perilla frutescens)花青素糖基转移酶基因为探针,通过电子克隆和RT-PCR的方法从金鱼草(Antirrhinum majus L.)叶片中克隆到花青素糖基转移酶基因(Am GT1)的全长c DNA,并对其表达特性进行分析。结果表明:Am GT1全长892 bp,编码277个氨基酸;进化分析表明Am GT1的氨基酸序列与白苏子的同源性最高为79%,与其他花青素糖基转移酶蛋白的同源性在42%~62%之间,表明Am GT1是从金鱼草中克隆的新的花青素糖基转移酶基因。实时定量RT-PCR分析表明:该基因在金鱼草叶片中的表达量最高,根中的表达量最低;该基因虽然在红色、粉色、黄色、白色花朵中均有表达,但在红色花朵中的表达量最高,且存在一个从紧蕾期到松蕾期的跃变。

Cloning and Expression Analysis of the AmGT1 Gene in Snapdragon

The full-length cDNA of the anthocyanin glycosyltransferase gene (AmGT1) in snapdragon (Antirrhinum majus L.) was isolated through in silico cloning and RT-PCR confirmation based the anthocyanin glycosyltransferase gene of the common perilla (Perilla frutescens) as the probe.The sequence and expression characteristics were further analysed.The full-length of AmGT1 was 892 bp,encoding 277 amino acids.Phylogenetic analysis showed that the sequence identity between AmGT1 and Perilla frutescens was 79％,displaying the highest consistency and sequence identity with other found GT proteins was between 42％-62％.These results indicated that AmGT1 was a new-found plant glycosyltransferase gene.Quantitative RT-PCR analysis revealed that the expression level of AmGT1 was the highest in the leaf and lowest in the root;AmGT1 was expressed in red,pink,yellow and white flowers;but the expression level of red flowers was the highest and there was a jump from tight bud stage to loose bud stage.