| 橡胶树褐根病菌的分子检测 |
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| 引用本文： | 梁艳琼,吴伟怀,李锐,郑肖兰,郑金龙,习金根,贺春萍,易克贤.橡胶树褐根病菌的分子检测[J].热带作物学报,2016,37(3):576-581. |
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| 作者姓名： | 梁艳琼 吴伟怀 李锐 郑肖兰 郑金龙 习金根 贺春萍 易克贤 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所 农业部热带农林有害生物入侵监测与控制重点开放实验室 海南省热带农业有害生物检测监控重点实验室 海南海口 571101 |
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| 基金项目： | 国家天然橡胶产业技术体系病虫害防控专家岗位项目（No. CARS-34-GW8）；中央级公益性科研院所基本科研业务费专项项目（No. 2014hzs1J013、No. 2015hzs1J014）。 |
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| 摘 要： | 利用褐根病菌的一对特异性引物G1F/G1R,对不同来源橡胶树褐根病菌菌株的r DNA-ITS区进行PCR扩增,对发病组织进行分子检测,并构建橡胶树褐根病菌系统发育树。结果表明:10株不同来源橡胶树褐根病菌菌株DNA均能扩增出一条653 bp的特异性片段,而橡胶树红根病菌、紫根病菌、臭根病菌、白根病菌和清水对照均无扩增条带。发病组织检测结果表明,该引物能特异性检测到橡胶树褐根病菌的存在。引物对基因组DNA检测灵敏度为100 ag/μL。基于r DNA-ITS系统发育分析结果表明,供试菌株与Gen Bank中的菌株KF233592、KM079591聚在同一分支上,其亲缘关系最近,均属于Phellinus noxius。
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| 关 键 词： | 橡胶树 褐根病菌 特异性引物 分子检测 |
Molecular Detection of Phellinus noxius in Rubber Tree |
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| LIANG Yanqiong,WU Weihuai,LI Rui,ZHENG Xiaolan,ZHENG Jinlong,XI Jingen,HE Chunping and YI Kexian.Molecular Detection of Phellinus noxius in Rubber Tree[J].Chinese Journal of Tropical Crops,2016,37(3):576-581. |
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| Authors: | LIANG Yanqiong WU Weihuai LI Rui ZHENG Xiaolan ZHENG Jinlong XI Jingen HE Chunping and YI Kexian |
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| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr;Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences/Key laboratory for Detection and Monitoring of Invasive Pest of Tropical, Agriculture and Forestry/Hainan Key Laboratory for Pests Detection and Monitoring of Tr |
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| Abstract: | |
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| Keywords: | Rubber tree Phellinus noxius Specificity primers Molecular detecting |
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