| 胡椒 PnNPR2 基因的克隆 |
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| 引用本文： | 范 睿,胡丽松,伍宝朵,郝朝运.胡椒 PnNPR2 基因的克隆[J].热带作物学报,2018,39(10):1983-1989. |
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| 作者姓名： | 范 睿 胡丽松 伍宝朵 郝朝运 |
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| 作者单位： | 1. 中国热带农业科学院香料饮料研究所，海南万宁 571533；
2. 农业部香辛饮料作物遗传资源利用重点实验室， 海南 万宁 571533；
3. 海南省热带香辛饮料作物遗传改良与品质调控重点实验室，海南万宁 571533 |
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| 摘 要： | 基于胡椒转录组数据库，筛选胡椒病程相关基因非表达子基因的 EST 序列，设计引物，运用 RACE 法克隆得 到 1 个 NPR 基因，命名为 PnNPR2；该基因全长 2 260 bp，开放阅读框 1 722 bp，编码 573 个氨基酸；开放读码框含有 BTB/POT 结构域、ANK 锚蛋白重复序列、DUF 和 NPR1-like C 4 个结构域，具有植物 NPR1 所共有的保守结构域，并 将 PnNPR2 基因插入 pCAMBIA1300-35S-sGFP 超表达载体中。本研究结果为 PnNPR2 的功能研究奠定基础。
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| 关 键 词： | 胡椒 NPR2 克隆 抗病 |
Cloning of PnNPR2 from Piper nigrum |
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| FAN Rui,HU Lisong WU Baoduo,HAO Chaoyun.Cloning of PnNPR2 from Piper nigrum[J].Chinese Journal of Tropical Crops,2018,39(10):1983-1989. |
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| Authors: | FAN Rui HU Lisong WU Baoduo HAO Chaoyun |
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| Institution: | 1. Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China;
2. Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture, Wanning, Hainan 571533, China;
3. Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China |
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| Abstract: | The full-length cDNA encoding nonexpressor of pathogenesis-related genes (NPR), designated PnNPR2, was isolated from black pepper by using RACE. The sequence of PnNPR2 was 2 260 bp in length, containing a 1 722 bp open reading frame, and encoding a polypeptide of 573 amino acids. The protein had conserved domains of BTB/POT, ANK anchored protein repeat sequences, DUF and NPR1-like C. The recombinant plasmid pCAMBIA1300-PnNPR2 was obtained by inserting the PnNPR2 gene into the pCAMBIA1300 vector. The results showed that the recombinant plasmid pCAMBIA1300-PnNPR2 which contained the target gene was constructed successfully. The result would generate an important theoretical and practical significance for black pepper genetic improvements. |
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| Keywords: | black pepper nonexpressor of pathogenesis-related genes 2 (NPR2) cloning disease resistance |
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