胡椒瘟病生防菌 Bacillus subtilis VD18R19 全基因组测序及 比较基因组学分析

Bacillus subtilis VD18R19 具有促进胡椒生长和防治胡椒瘟病的效果，全基因组测序是其分子机理研究和开发 利用的重要基础。本研究采用第二代 Illumina 平台与第三代 PacBio 平台相结合的测序技术，对生防菌 VD18R19 进行 全基因组测序，并进行比较基因组学分析。结果发现，VD18R19 全基因组大小为 4 123 380 bp，GC 含量为 43.80%， 编码基因 4 245 个；含有 tRNA 85 个、rRNA 30 个、sRNA 33 个；含有串联重复序列 60 个，其中小卫星 DNA 43 个、 微卫星 DNA 1 个；共线性分析、core-pan 基因分析及基因家族分析结果均显示 VD18R19 与模式菌株 B. subtilis 168 具 有高度的同源性；antiSMASH 软件预测及同源序列比对结果显示，VD18R19 菌株中含有 6 个抑菌次生代谢产物合成基 因簇，编码 surfactin、plipastatin、bacillibactin、bacilysin、bacillaene、subtilosin A 等抑菌物质，其合成途径涵盖了核 糖体途径、非核糖体途径和聚酮合酶途径。本研究为深入研究生防菌 VD18R19 的分子机理奠定生物信息学基础，有利 于生防菌株及其抑菌次生代谢产物的开发和利用。

Whole Genome Sequencing and Comparative Genomics Analysis of Bacillus subtilis VD18R19 with Biocontrol Activity Against Pepper Phytophtora Rot Disease

Bacillus subtilis VD18R19 has beneficial effects on pepper growth-promoting and bio-control against pepper Phytophtora rot disease. The whole genome sequencing is an important basis for further molecular research and application. The genome was sequenced using a PacBio RS II platform and Illumina HiSeq 4000 platform, and the gene function annotation and comparative genomics analysis were performed using blast alignment tools. The genome is 4 123 380 bp with GC content of 43.80%, 4 245 identified genes, 85 tRNA, 30 rRNA, 33 sRNA, 60 tandem repeat regions including 43 minisatellite DNA and 1 microsatellite DNA. The genome showed highly homologous with the model strain B. subtilis 168 by the analysis of genome synteny, core-pan gene and gene family. It harbors six antibiotic gene clusters, directing synthesis of surfactin, plipastatin, bacillibactin, bacilysin, bacillaene and subtilosin A, through ribosomal synthetases (RS) pathway or non-ribosomal peptide synthetases (NRPS) pathway or polyketide synthases(PKS) pathway. This study would provide a database for further research of diverse molecular biology mechanisms and is helpfull in utilizing the beneficial strain and the functional antibiotics.