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| 44份香料烟品种ISSR遗传多样性分析 | |
| 引用本文： | 张晨东,杨志新,张聪,杨懋杰,罗建蓉,陈学军.44份香料烟品种ISSR遗传多样性分析[J].热带作物学报,2012,33(12):2144-2149. |
| 作者姓名： | 张晨东 杨志新 张聪 杨懋杰 罗建蓉 陈学军 |
| 作者单位： | 1. 云南省烟草农业科学研究院,云南玉溪,653100 2. 云南农业大学资源与环境学院,云南昆明,650201 3. 河南省烟草公司镇平县公司,河南镇平,474250 4. 云南省烟草公司龙陵县公司,云南龙陵,678300 5. 云南大学旅游文化学院,云南丽江,674100 |
| 基金项目： | 中国烟草总公司科技项目及中国烟草总公司云南省公司科技项目资助（No. 110201102001；No. 2011YN07）。 |
| 摘 要： | 以44份香料烟品种资源为材料,利用ISSR分子标记技术进行了遗传多样性分析.结果表明:100个ISSR引物中可筛选出8个核心引物,在44份香料烟品种中扩增出223个片段,其中209个具多态性.供试香料烟品种的遗传相似系数在0.51～0.96之间,平均为0.79.当相似系数为0.636时,44份香料烟品种可分为两大类,表明44份香料烟品种间的遗传多样性低,急需通过引种及种质创新等手段来丰富香料烟品种的遗传多样性. |
| 关 键 词： | 香料烟 ISSR 遗传多样性 |
ISSR Analysis of Genetic Diversity for 44 Oriental Tobacco Cultivars |
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| ZHANG Chendong,YANG Zhixin,ZHANG Cong,YANG Maojie,LUO Jianrong and CHEN Xuejun.ISSR Analysis of Genetic Diversity for 44 Oriental Tobacco Cultivars[J].Chinese Journal of Tropical Crops,2012,33(12):2144-2149. | |
| Authors: | ZHANG Chendong YANG Zhixin ZHANG Cong YANG Maojie LUO Jianrong and CHEN Xuejun |
| Abstract: | ISSR analysis was conducted to estimate the degree of genetic diversity for the 44 oriental tobacco cultivars. The results showed that the 8 primers, which selected from 100 ISSR primers, generated 223 fragments, of which 209 fragments were polymorphic. The genetic similarity coefficient among all materials was distributed from 0.51 to 0.96, average 0.79. The 44 oriental tobacco cultivars was divided into 2 types by a similarity coefficient 0.636. Two major distinct clusters were discovered by using UPGMA methods. And great efforts should be made to broaden genetic diversity of oriental tobacco by germplasm introduction and innovation. |
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