| 拮抗放线菌菌株FS-4发酵工艺筛选 |
| |
| 引用本文： | 段雅婕,梅志刚,孙德权,李伟明,庞振才,胡会刚.拮抗放线菌菌株FS-4发酵工艺筛选[J].热带作物学报,2020,41(2):339-345. |
| |
| 作者姓名： | 段雅婕 梅志刚 孙德权 李伟明 庞振才 胡会刚 |
| |
| 作者单位： | 1. 中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室,广东湛江 5240912. 广东海洋大学机械与动力工程学院,广东湛江 524088 |
| |
| 基金项目： | 国家香蕉产业技术体系湛江试验站项目(CARS-31-16);海南省自然科学基金(318MS099);现代农业人才支撑计划(2130106) |
| |
| 摘 要： | FS-4是1株对香蕉枯萎病菌有较好拮抗作用的放线菌。以高氏一号培养基为基础,通过单因素和响应面实验进行了相关优化,对FS-4菌株的发酵工艺进行筛选。结果表明：0.5%蛋白胨,2.4%蔗糖,0.05%的磷酸氢二钾、氯化钠和硫酸镁,发酵温度28 ℃,初始pH为7为最佳培养基配方及最优发酵条件。发酵62 h后,发酵液中抑菌物质活性达到最高水平,对香蕉枯萎病菌的抑菌圈直径达到27.1 mm。
|
| 关 键 词： | 放线菌菌株FS-4 发酵工艺 响应面法 |
| 收稿时间： | 2019-04-02 |
Optimization of Fermentation Conditions of Antagonistic Actinomycetes FS-4 |
| |
| DUAN Yajie,MEI Zhigang,SUN Dequan,LI Weiming,PANG Zhencai,HU Huigang.Optimization of Fermentation Conditions of Antagonistic Actinomycetes FS-4[J].Chinese Journal of Tropical Crops,2020,41(2):339-345. |
| |
| Authors: | DUAN Yajie MEI Zhigang SUN Dequan LI Weiming PANG Zhencai HU Huigang |
| |
| Institution: | 1. South Asia Tropical Crops Research Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Tropical Fruit’s Biology, Ministry of Agriculture and Rural Affairs, Zhanjiang, Guangdong 524091, China2. School of Mechanical and Power Engineering, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China |
| |
| Abstract: | The screening of antagonistic bacteria from soil microorganisms in the areas with banana Fusarium wilt is of great practical significance for controlling this disease. In this report, a strain, designated FS-4, was isolated from healthy banana rhizosphere soil in the area affected by Fusarium wilt. Based on Gause’s No. 1 synthetic medium, the optimization of medium, single factor experiments and response surface experiments were conducted to maximize the production of the antibacterial substances of actinomycete FS-4. The best fermentation medium and fermentation conditions were as follows: sucrose 2.4%, peptone 0.5%, K2HPO4 0.05%, NaCl 0.05%, MgSO4 0.05%, fermentation temperature 28 ℃, initial pH 7. Under the conditions, the inhibition zone diameter of actinomycete FS-4 fermentation filtrate reached 27.1 mm on the test plates of Bacillus subtilis after 62 h incubation |
| |
| Keywords: | actinomycetes FS-4 fermentation conditions response surface method |
|
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
