| 花粉管通道法转化荔枝的初步研究 |
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| 引用本文： | 王树军,孙进华,李焕苓,王果,李芳,王家保.花粉管通道法转化荔枝的初步研究[J].热带作物学报,2020,41(2):252-257. |
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| 作者姓名： | 王树军 孙进华 李焕苓 王果 李芳 王家保 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,海南海口 571101 |
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| 基金项目： | 海南省自然科学基金项目(319QN270);国家现代农业产业技术体系(CARS-32-03) |
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| 摘 要： | 为开拓荔枝转基因育种新途径,以GUS基因作为报告基因,用花粉管通道法转化授粉后24 h的‘新球蜜荔’荔枝雌花,并统计座果率、成苗率、转化率。结果显示：共获得303株实生苗,经PCR检测和GUS染色法证实外源基因整合到4株荔枝苗基因组中,转化率为1.32%。此研究结果为荔枝生物技术育种提供了一种简单有效的途径。
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| 关 键 词： | 荔枝 花粉管通道 转基因 育种 |
| 收稿时间： | 2019-01-10 |
Gene Transformation of Litchi via Pollen Tube Pathway |
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| WANG Shujun,SUN Jinhua,LI Huanling,WANG Guo,LI Fang,WANG Jiabao.Gene Transformation of Litchi via Pollen Tube Pathway[J].Chinese Journal of Tropical Crops,2020,41(2):252-257. |
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| Authors: | WANG Shujun SUN Jinhua LI Huanling WANG Guo LI Fang WANG Jiabao |
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| Institution: | Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China |
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| Abstract: | In order to develop a new gene transformation pathway for litchi, the female flowers of Xinqiumili (24 h after pollination) were transformed by the pollen tube pathway method, using GUS as a report gene, and the fruit set percentage, seeding rate, and conversion rate were counted. A total number of 303 seedlings were obtained, and four positive transformed plants were verified according to PCR analysis and GUS staining, the transformation rate was 1.32%. This study would provide a simple and efficient way for the biotechnology breeding of litchi. |
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| Keywords: | litchi pollen tube path transgenic breeding |
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