三相分离法提取胡椒油树脂的工艺研究

本文以黑胡椒粉为原料,采用三相分离法（TPP）提取胡椒油树脂,研究了胡椒粉添加量、硫酸铵质量浓度、提取液与叔丁醇体积比、pH、温度对胡椒油树脂得率及胡椒碱含量的影响,并通过响应面试验优化了胡椒油树脂的提取工艺。结果表明：最佳提取条件为胡椒粉添加量5%、硫酸铵质量浓度10%、提取液与叔丁醇的体积比1∶0.5、pH 4、温度20℃,在此条件下,黑胡椒油树脂得率为12.90%,黑胡椒油树脂中胡椒碱含量为30.46%,验证试验与模型预测值基本相符。研究结果为工业化提取胡椒油树脂提供参考。

Processing Parameters of Extracting Pepper Oleoresin by Three Phase Partitioning

In this study, three phase partitioning (TPP) was employed to extract the pepper oleoresin from black pepper powder. The effects of the addition amount of pepper powder, ammonium sulphate concentration, ratio of crude extract to t-butanol, pH and temperature on the yield of pepper oleoresin and piperine content were studied. In addition, the extraction process of pepper oleoresin was optimized by a response surface test. The optimal condition obtained was as follow: the addition amount of pepper powder 5%, ammonium sulphate concentration 10%, ratio of crude extract to t-butanol 1∶0.5, pH 4 and temperature 20℃. Under the optimal conditions, the yield of black pepper oleoresin and piperine content was 12.90% and 30.46%, respectively. The results provide a reference for the industrial extraction of pepper oleoresin.