海南甘薯主栽品种再生体系的优化

本研究以海南甘薯主栽品种‘心香’、‘广薯87’、‘川山紫’、‘宁紫薯1号’、‘薯绿一号’、‘高系14’和‘三角宁’为材料,从外植体的选择、不同消毒方案、培养基配方和防褐化剂筛选等方面进行研究,建立一套适合热带地区甘薯主栽品种的脱毒快繁优化再生体系。结果表明：侧芽增殖率与存活率最高;在不同的外植体消毒处理中,依次用75%酒精消毒60 s,2% NaClO消毒15 min,以及0.1% HgCl₂消毒15 min的污染率最低;最佳的生根培养基配方为：MS+0.05 mg/L NAA+0.1 mg/L GA₃;茎长生长最快的培养基配方为：MS+0.1 mg/L NAA+1 mg/L 6-BA+0.5 mg/L GA₃;干重增长最快的培养基配方为：MS+0.1 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA₃;在培养基中加入5~6 g/L硫代硫酸钠和1.25 g/L聚乙烯吡咯烷酮能有效地抑制外植体的褐变。

Optimization of Regeneration System of Main Sweet Patato Cultivars in Hainan,China

In this study, the main sweet potato varieties planted in tropical areas, including ‘Xinxiang’, ‘Guangshu 87’, ‘Chuanshan Zi’, ‘Ning Zishu 1’, ‘Shulv 1’, ‘Gaoxi 14’ and ‘Sanjiao Ning’ were used as the materials. The selection of explants, disinfection schemes, medium formulation and anti-browning agent were explored. The optimum regeneration system of virus-free and rapid propagation was established for the main sweet potato varieties. The proliferation rate and survival rate of lateral buds were the highest among different parts of explants. In treatments of different explant disinfection, 75% alcohol for 60 s, 2% sodium hypochlorite for 15 min, and 0.1% mercury chloride for 15 min exhibited the lowest contamination rate. The medium formula with the shortest rooting time was as follows: MS+0.05 mg/L NAA+0.1 mg/L GA₃. The medium formula with the fastest growth of stem length was as follows: MS+0.1 mg/L NAA+1 mg/L 6-BA+0.5 mg/L GA₃. The medium formula with the fastest growth rate of dry weight was as follows: MS+0.1 mg/L NAA+1.5 mg/L 6-BA+0.5 mg/L GA₃. Adding 5-6 g/L sodium thiosulfate and 1.25 g/L polyvinylpyrrolidone to the medium could effectively inhibit the browning of explants.