| 茶树鲜叶抗坏血酸过氧化物酶活性的变化规律及测定方法 |
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| 引用本文： | 孙云,江春柳,赖钟雄,邵巍,王秀英.茶树鲜叶抗坏血酸过氧化物酶活性的变化规律及测定方法[J].热带作物学报,2008,29(5):562-566. |
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| 作者姓名： | 孙云 江春柳 赖钟雄 邵巍 王秀英 |
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| 作者单位： | 1. 福建农林大学园艺植物生物工程研究所,福州,350002;福建农林大学园艺学院福州,350002
2. 福建农林大学园艺植物生物工程研究所,福州,350002 |
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| 基金项目： | 福建省果茶重大科技专项(
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| 摘 要： | 探讨茶树鲜叶抗坏血酸过氧化物酶(APX)活性的测定条件,分析不同茶树品种以及茶树鲜叶不同叶位APX活性的变化.结果表明:聚乙烯聚吡咯烷酮(PVPP)加入量约为鲜叶重的1.5倍,酶提取液pH为7.8.反应液pH为7.O,抗坏血酸(AsA)浓度为0.50 mmol/L时茶树鲜叶APX活性最高;茶树鲜叶APX活性随冷藏时间的延长呈下降趋势;福建7个主要茶树品种以福鼎大白茶的APX活性最高,铁观音的APX活性最低;茶树鲜叶不同叶位APX活性变化趋势为:芽>第一叶>第二叶>第三叶>老叶>嫩茎.
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| 关 键 词： | 茶树 鲜叶 抗坏血酸过氧化物酶(APX) 酶活性 测定方法 |
Determination and Observation of the Changes of the Ascorbate Peroxidase Activities in the Fresh Leaves of Tea Plants |
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| Sun Yun,Jiang Chunliu,Lai Zhongxiong,Shao Wei and Wang Xuiyin.Determination and Observation of the Changes of the Ascorbate Peroxidase Activities in the Fresh Leaves of Tea Plants[J].Chinese Journal of Tropical Crops,2008,29(5):562-566. |
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| Authors: | Sun Yun Jiang Chunliu Lai Zhongxiong Shao Wei and Wang Xuiyin |
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| Institution: | 1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;2.College of Horticulture, Fujian Agriculture and Forestry University;1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;2.College of Horticulture, Fujian Agriculture and Forestry University;1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;2.College of Horticulture, Fujian Agriculture and Forestry University;Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;1.Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University;2.College of Horticulture, Fujian Agriculture and Forestry University |
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| Abstract: | The ascorbate peroxidase activities in the fresh leaves of tea plants were determined and their optimum conditions for determination discussed. The changes of the ascorbate peroxidase activities in the different tea cultivars and on the different leaf positions of the fresh leaf were observed. The ascorbate peroxidase activities in the tea fresh leaves were the highest when determined under the conditions that the weight of PVPP added was 1.5 times of the weight of fresh leaf, that the pH was 7.8 for extraction solution and 7.0 for reaction solution and, that the AsA concentration was 0.50mmol/L. The ascorbate peroxidase activities in tea fresh leaves showed a tendency to decrease along with cold storage duration. The tea Fuding Dabaicha was the highest in ascorbate peroxidase activities among 7 major tea cultivars in Fujian Province, while Tieguanyin was the lowest. The ascorbate peroxidase activities in the tea changed with leaf positions in the order of the bud > the first leaf> the second leaf> the third leaf> the old leaf >the young stem. |
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| Keywords: | tea fresh leaf ascorbate peroxidase enzymatic activity determination |
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