猴耳环多酚的大孔树脂纯化工艺及其组分分析

建立并优化猴耳环多酚的纯化工艺并对其组分进行定性分析。本研究以猴耳环多酚粗提物为原料,以吸附及解吸附效果筛选了最优大孔树脂,再以吸附、解吸附和纯度为评价指标,考察各工艺参数对AB-8树脂纯化多酚的影响,最后采用超高效液相色谱-四级杆-飞行时间串联质谱（UPLC-Q-TOF-MS）法进行多酚组成定性分析。结果表明：优选 AB-8树脂,粗提物以质量浓度3 mg/mL、流速1 mL/min上样10 BV（柱体积：1 BV=10 mL）,水洗后,用60%乙醇5 BV以1 mL/min的流速洗脱。在此工艺条件下,多酚纯度达到58.64%。UPLC-Q-TOF-MS定性分析了纯化后猴耳环多酚14种组分。建立的猴耳环多酚的纯化工艺稳定可行,实现了其组分定性分析,为进一步研究提供了依据。

Macroporous Resin Purification Process and Component Analysis of Pithecellobium clypearia Polyphenols

The aim of the study was to establish and optimize the purification process of Pithecellobium clypearia polyphenols and qualitatively analyze its components. In this study, the crude polyphenol extract of P. clypearia was used as the raw material, and the optimal macroporous resin was screened based on the adsorption and desorption effects. Taking adsorption, desorption and purity as the evaluation indexes, the influence of various technological parameters on the purification of polyphenols by AB-8 resin was investigated. Finally, the ultra-high performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS) method was used for qualitative analysis of polyphenol composition. AB-8 resin was preferred, the crude extract was loaded with 10 BV at a mass concentration of 3 mg/mL and a flow rate of 1 mL/min (column volume: 1 BV=10 mL), after washing with water, eluted with 60% ethanol 5 BV at a flow rate of 1 mL/min. Under this process condition, the purity of polyphenol reached 58.64%. UPLC-Q-TOF-MS qualitatively analyzed 14 components of purified. The purification process of the established P. clypearia polyphenols was stable and feasible, and the qualitative analysis of its components was realized, which would provide a basis for further research.