| 龙眼DlTRF2-like的克隆及表达模式分析 |
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| 引用本文： | 李浩然,张树伟,王金英,黄小雄,彭宏祥,徐炯志,潘介春,朱建华,谭春露,李平,丁峰.龙眼DlTRF2-like的克隆及表达模式分析[J].热带作物学报,2021,42(12):3471-3476. |
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| 作者姓名： | 李浩然 张树伟 王金英 黄小雄 彭宏祥 徐炯志 潘介春 朱建华 谭春露 李平 丁峰 |
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| 作者单位： | 1.广西壮族自治区农业科学院/广西作物遗传改良生物技术重点开放实验室,广西南宁 5300072.广西壮族自治区农业科学院园艺研究所,广西南宁 5300073.广西大学农学院/亚热带农业生物资源保护与利用国家重点实验室,广西南宁 5300044.桂平麻垌镇农业技术推广站,广西桂平 537200 |
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| 基金项目： | “广西八桂青年学者”专项经费(2019AQ01);广西农业科学院基本科研业务专项(桂农科)(2021YT124);广西作物遗传改良重点实验室开放课题(20-065-39-01-05) |
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| 摘 要： | 以龙眼成熟叶片为材料,克隆龙眼MYB-related基因家族TRF2-like基因,命名为DlTRF2-like,并对其进行生物信息学和表达模式分析。DlTRF2-like基因属于MYB-related家族的TRF-like亚家族,ORF长度是909 bp,编码含有302个氨基酸残基的蛋白,预测该基因定位于细胞核,同源基因进化分析表明龙眼DlTRF2-like与阿月浑子PvTRF2-like亲缘关系最近。利用实时荧光定量PCR检测DlTRF2-like基因在‘四季蜜’龙眼不同组织及乙烯利和多效唑处理后不同时间的相对表达量。结果表明：DlTRF2-like在龙眼不同组织中均有表达,在花芽中表达量最高;乙烯利和多效唑处理后,DlTRF2-like基因的表达量明显高于对照,推测DlTRF2-like响应乙烯利和多效唑信号促进龙眼成花。
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| 关 键 词： | 龙眼 MYB-related 龙眼成花 TRF-like亚家族 表达模式分析 |
| 收稿时间： | 2021-04-09 |
Cloning and Expression Pattern Analysis of DlTRF2-like in Longan |
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| LI Haoran,ZHANG Shuwei,WANG Jinying,HUANG Xiaoxiong,PENG Hongxiang,XU Jiongzhi,PAN Jiechun,ZHU Jianhua,TAN Chunlu,LI Ping,DING Feng.Cloning and Expression Pattern Analysis of DlTRF2-like in Longan[J].Chinese Journal of Tropical Crops,2021,42(12):3471-3476. |
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| Authors: | LI Haoran ZHANG Shuwei WANG Jinying HUANG Xiaoxiong PENG Hongxiang XU Jiongzhi PAN Jiechun ZHU Jianhua TAN Chunlu LI Ping DING Feng |
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| Institution: | 1. Guangxi Academy of Agricultural Sciences / Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, Guangxi 530007, China2. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007, China3. College of Agriculture, Guangxi University / State Key Laborartory for Coservation and Utilizaztion of Subtropical Agro-bioresources, Nanning, Guangxi 530004, China4. Agricultural Technology Extension Station of Madong, Guiping, Guangxi 537200, China |
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| Abstract: | The TRF2-like gene of MYB-related family was cloned from the mature leaves of longan and named DLTRF2-like. Bioinformatics and the expression pattern of DLTRF2-like were analyzed. DLTRF2-like gene belongs to the TRF-like subfamily of MYB-related family, with an ORF length of 909 bp, encoding a protein of 302 amino acids which was predicted to be located in the nucleus. Evolution analysis of homologous genes indicated that DLTRF2-like was most closely related to PvTRF2-like in Pistacia vera L. The expression pattern of DLTRF2-like in different tissues and leaves treated by ethephon and paclobutrazol was analyzed by real-time quantitative PCR. The results showed that DLTRF2-like was expressed in all the samples, and the highest expression level was found in flower buds. The expression level of DLTRF2-like gene in the leaves treated by ethephon and paclobutrazol was significantly higher than that of the control. The results indicated that DLTRF2-like might response to ethephon and paclobutrazol signal to promote the flowering of longan. |
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| Keywords: | longan MYB-related longan flowering TRF-like subfamily expression pattern analysis |
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