胡椒不同时期体细胞胚中内源激素变化

为研究胡椒不同时期体细胞胚中内源激素变化,以‘热引1号’胡椒外种皮经培养获得的非胚性愈伤组织（Y）、胚性愈伤组织（P）、胚性愈伤组织与体细胞胚混合物（H）、体细胞胚（T）与体细胞胚增殖（Z）5个时期样品为试验材料,采用LC-MS/MS技术检测不同时期胚性培养物的内源激素种类与含量,以期找出内源激素影响胡椒体细胞胚发生的关键因素。结果表明：在胡椒体细胞胚发生过程中,吲哚-3-乙酸（IAA）含量在H时期最高,在P时期最低,在H、T与Z时期逐渐降低。吲哚-3-丁酸（IBA）含量在Y时期最高,在P时期最低,在H、T与Z时期基本不变。吲哚-3-甲酸（ICA）含量在H时期最高,且只存在含有愈伤组织的Y、P与H时期。异戊烯腺嘌呤（IP）含量在H时期最高,在P时期最低,在T与Z时期接近于零。顺式玉米素（cZ）含量在Y时期最高,其他时期都接近于零。茉莉酸甲酯（ME-JA）含量只存在Y时期和P时期,但Y时期含量高于P时期。茉莉酸（JA）含量在Z时期最高,在P时期最低,在H、T与Z时期逐渐升高。水杨酸（SA）含量在H时期最高,在Y时期最低,且H、T与Z时期先降后升。脱落酸（ABA）含量在H时期最高,在P时期最低,在T与Z时期基本不变。赤霉素类（GA_S）主要存在于Y与P时期,H、T与Z时期含量很低。由此可见,IAA、SA与ABA可能与体细胞胚发生的相关性强,而cZ、MEJA与GA_S可能与愈伤组织产生相关性强。

Changes of Endogenous Hormone During Different Stages of Somatic Embryogenesis of Piper nigrum

In order to investigate the changes of endogenous hormones during different stages of somatic embryogenesis of P. nigrum, the endogenous hormones in five different development processes were detected at non-embryonic callus stage (Y), embryonic callus stage (P), mixed stage of embryonic callus and somatic embryo (H), somatic embryo stage (T) and stage of somatic embryo proliferation (Z) by the LC-MS/MS technique. The content of indole-3-acetic acid (IAA) decreased gradually in the period of H, T and Z during the process of pepper somatic embryogenesis. The content of indole-3-butyric acid (IBA) was the highest in the period of Y and the lowest in the period of P, and the content of IBA was basically unchanged in the period of H, T and Z. Indole-3- Formic acid (ICA) content was the highest in the period of H, and ICA only existed at callus stage. Isopentenyladenine (IP) content was the highest at H, and was the lowest at P and close to zero at T and Z. Zeatin (cZ) content was the highest at Y, and cZ content was close to zero at other stages. Methyl jasmonate (ME-JA) only existed at Y and P, and former content was higher than latter content. Jasmonic acid (JA) content was the highest at Z, JA content was the lowest at P and increased gradually at other stages. Salicylic acid (SA) content was the highest at H, and was the lowest at Y and increased after decreased at other stages. Abscisic acid (ABA) content was the highest at H, and was the lowest at P and unchanged basically at T and Z. Gibberellins (GA_S) mainly existed at Y and P, the levels were low at H, T, and Z. Therefore, IAA, SA and ABA may be strongly correlated with somatic embryogenesis, while cZ, ME-JA and GA_S may be strongly correlatws with callus production.