带叶兜兰成花过程中叶片内源激素与多胺含量的变化动态

近些年兜兰产业发展迅速,但花期调控难成为限制兜兰产业发展的瓶颈。以带叶兜兰为试验材料,采用高效液相色谱分析的方法,研究带叶兜兰开花期间叶片中内源激素及多胺含量的变化规律,分析内源激素及多胺对其开花的影响。结果表明,高水平的ZT、IAA、GA₃、多胺含量和GA₃/IAA比值有利于带叶兜兰花芽分化;带叶兜兰花朵形态建成需要更高水平的ZT、IAA、GA₃、多胺含量和Spd/Put、Spm/Put比值;带叶兜兰叶片GA₃具有调控IAA水平和Put水平的作用,多胺和激素之间存在密切的关系。

Dynamic Changes of Endogenous Hormones and Polyamine in Paphiopedilum hirsutissimum Leaves During Floral Bud Formation

The Paphiopedilum orchid industry has developed rapidly recently. However, flowering regulation has become the bottleneck restricting the development of the industry. P. hirsutissimum, which is easy to bloom, was used as the experiment materials, the content changes of endogenous hormones and polyamine in functional leaves were detected by high performance liquid chromatography (HPLC) to provide a theoretical basis for the flowering regulation of the orchids. High content of ZT, IAA, GA₃, PAs and high ratio of GA₃/IAA in leaves were beneficial to inducing the bud differentiation. Higher content of ZT, IAA, GA₃, PAs and high ratio of Spd/Put, Spm/Put in leaves were beneficial to floral morphological differentiation. GA₃ could regulate IAA and Put levels, there was a close relationship between polyamines and hormones.