海南17a宿根巴西蕉园土壤微生物特征及土壤pH周年变化特征分析

香蕉是全球性的大宗经济水果,我国是香蕉生产大国也是消费大国,但产量和品质不能满足需求,每年仍需大量进口.香蕉枯萎病是影响我国香蕉产能的主要因素.巴西蕉在我国曾广泛种植,但易感香蕉枯萎病,罕有宿根蕉园.宿根蕉园栽培模式在成本控制、产期调节、环境友好等方面有巨大优势.前期研究结果显示,土壤pH以及土壤菌群与香蕉枯萎病的发生...

Soil Microbial Flora and pH Annual Change Characteristics of 17 Years Continuous Cropping Banana Field in Hainan,China

Banana is one of the most widely planted fruits in the world and is a staple for millions of people worldwide. China is a large banana production country but still needs to import huge amounts of banana to meet the demand. Brazil banana has been widely planted in China but it is very susceptible to Fusarium wilt (Panama disease). Ratoon banana filed (RTF) is common in foreign country and famous for its low cost, easy production period regulation and environmental friendliness. RTF is seldom used in China. In this study, the microbial community structure of 17-year Lingao RTF banana field was characterized through ITS and 16S rRNA gene sequencing and the reclaim banana field (RCF) and banana cane rotation field (BCF) nearby were used as the control. The results showed that the diversity and abundance of soil bacteria and fungi in RTF were significantly higher than that of RCF and BCF. The classification of soil microbial species showed that RTF were significantly different among 11 bacteria and 6 fungal taxonomic units such as Agrobacterium tumefaciens, actinomycetes and so on. RTF soil characteristic microorganism showed significantly different with RCF and BCF in Micromonospora, Bacillus and Ceratobasidium. The functional abundance heat map showed that the soil bacterial activity in RTF was significantly lower than that of RCF and BCF, while the fungal activity was significantly higher. Gene abundance analysis of metabolic pathways showed that soil bacteria in RCF and BCF were significantly more active in biosynthesis and secondary metabolism, transcription, carbon metabolism, membrane transfer and other pathways, while soil fungi in RTF was significantly more active in cell migration, cell growth and death, signal transduction, nucleic acid metabolism, protein folding, storage, transportation and degradation. Through the analysis of soil microbial characteristics of RTF, it would lay a foundation for clarifying the internal mechanism of banana ratoon model, and also provide a new reference for the renew of abandoned banana garden.