甘蔗渣对灵芝菌丝生长的影响及菌种保藏评价

为了筛选出最适合灵芝菌种复壮的培养基和灵芝菌种的保藏方法,通过添加不同含量的甘蔗渣提取液作为灵芝菌株复壮的培养基,观察灵芝菌丝在不同培养基下的生长速度和生长状况,并通过对灵芝菌种酶活力进行测定分析,比较几种不同保藏方法对灵芝菌种活力的影响。结果表明：马铃薯葡萄糖培养基（PDA）培养基添加甘蔗渣提取液能更好促进灵芝菌丝生长,添加100 g/L甘蔗渣提取液,赤灵芝GL102、紫灵芝GS63和GS15菌丝生长速率分别提高48%、70%和28%。与PDA保藏和石蜡油保藏培养基相比,甘蔗渣保藏的灵芝菌丝生长快、长势好,脱氢酶活力高。因此,甘蔗渣可用于灵芝菌种的复壮和保藏。

Effect of Bagasse on Growth of Ganoderma Mycelium and Evaluation of Strain Preservation

In order to select the most suitable culture medium for the rejuvenation and preservation method of Ganoderma strains, by adding different contents of bagasse extract as the medium for the rejuvenation and observed hypha growth rate under different culture medium and growth conditions, and through the comparison of several different preservation methods on the vigor and the analysis of the enzyme activity. The results showed that the mycelium growth was better promoted by adding extraction solution of bagasse into PDA medium, and the mycelium growth rate of Ganoderma lucidum GL102, Ganoderma sinensis GS63 and GS15 increased by 48%, 70% and 28% respectively with the addition of 100 g/L bagasse extract. Compared with PDA preservation and paraffin oil preservation medium, bagasse preservation of Ganoderma mycelia grew fast, grew well and had high dehydrogenase activity. It was concluded that bagasse can be used for the rejuvenation and preservation of Ganoderma strains.