| 猪链球菌细胞外蛋白因子Ef基因的克隆与原核表达 |
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| 引用本文： | 丛 茜,潘晓磊,韦双双,郑继平.猪链球菌细胞外蛋白因子Ef基因的克隆与原核表达[J].热带作物学报,2010,34(4):32-35. |
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| 作者姓名： | 丛 茜 潘晓磊 韦双双 郑继平 |
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| 作者单位： | 海南大学农学院;海南大学农学院;海南大学农学院;海南大学农学院 |
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| 摘 要： | 采用分子克隆手段，将Ef全长基因克隆至表达载体pGEX-6p-1的Ptac启动子下游，电击转入大肠杆菌E.cohi BL21(DE3)中表达。经PCR扩增和蛋白质凝胶电泳鉴定，表明该EF蛋白获得有效表达，为进一步构建猪链球菌Ⅱ生物工程疫苗提供前期载体。
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| 关 键 词： | 猪链球菌细胞外蛋白因子 Ef基因 克隆 原核表达 |
Cloning and Expression of the Extracellular Protein factor of Streptococcus suis |
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| CONG Qian,PAN Xiaolei,WEI Shuangshuang and ZHENG Jiping.Cloning and Expression of the Extracellular Protein factor of Streptococcus suis[J].Chinese Journal of Tropical Crops,2010,34(4):32-35. |
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| Authors: | CONG Qian PAN Xiaolei WEI Shuangshuang and ZHENG Jiping |
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| Institution: | College of Agronomy, Hainan University;College of Agronomy, Hainan University;College of Agronomy, Hainan University;College of Agronomy, Hainan University |
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| Abstract: | By molecular cloning method, full-length gene Ef was cloned into the downstream of the Ptac promoter of expression vector pGEX-6p-1, electric shock transformed into E. cohi BL21 (DE3) expression. After PCR amplification, and protein gel electrophoresis, the EF protein was effectively expressed, providing further construction of bio-engineering vaccine of Streptococcus suis serotype 2 with an initial vector. |
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