| 中国橡胶树苗圃2种炭疽病菌分子鉴定及分布分析 |
| |
| 引用本文： | 林春花,孙董董,韩丹,黄贵修.中国橡胶树苗圃2种炭疽病菌分子鉴定及分布分析[J].热带作物学报,2014,35(9):1802-1808. |
| |
| 作者姓名： | 林春花 孙董董 韩丹 黄贵修 |
| |
| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所 海南省热带农业有害生物检测监控重点实验室 海南海口571101
2. 中国热带农业科学院环境与植物保护研究所 海南省热带农业有害生物检测监控重点实验室 海南海口571101;华中农业大学植物科学技术学院,湖北武汉430070 |
| |
| 基金项目： | 国家天然橡胶产业技术体系资金资助项目（No. Cars-34-GW8）；中国热带农业科学院院本级基本科研业务费项目(No. 1630042014001)。 |
| |
| 摘 要： | 根据胶孢炭疽菌和尖孢炭疽菌特异引物对,对采自中国云南、海南、广东和广西植胶区的138株炭疽病菌进行分子鉴定。结果显示:138株炭疽菌中鉴定为胶孢炭疽菌的有100株,占总测定株数的72.46%,尖孢炭疽菌的有38株,占总测定株数的27.54%。其中海南、云南和广东橡胶苗圃中胶孢炭疽菌占所分析菌株总数比例分别为81.82%(54/66)、60.0%(15/25)和77.27%(17/22),而广西3个橡胶苗圃中的2个苗圃地尖孢炭疽和胶孢炭疽病菌各占一半。总体来看,国内橡胶树苗圃地炭疽病病原菌仍以胶孢炭疽菌为主,部分苗圃同时存在胶孢和尖孢炭疽菌的危害。
|
| 关 键 词： | 橡胶树 胶孢炭疽 尖孢炭疽 种特异性PCR 分子鉴定 |
Identification of Colletotrichum Species on the Colletotrichum Leaf Disease from Rubber Nurseries in China |
| |
| LIN Chunhu,SUN Dongdong,HAN Dan and HUANG Guixiu.Identification of Colletotrichum Species on the Colletotrichum Leaf Disease from Rubber Nurseries in China[J].Chinese Journal of Tropical Crops,2014,35(9):1802-1808. |
| |
| Authors: | LIN Chunhu SUN Dongdong HAN Dan and HUANG Guixiu |
| |
| Institution: | Hainan Provincial Key Laboratory for Baleful Biology Detection and Monitoring of Tropical Agriculture / Environment and Plant Protection Institute, CATAS;1 Hainan Provincial Key Laboratory for Baleful Biology Detection and Monitoring of Tropical Agriculture / Environment and
Plant Protection Institute, CATAS 2 College of Plant Science and Technology, Huazhong Agricultural University;Hainan Provincial Key Laboratory for Baleful Biology Detection and Monitoring of Tropical Agriculture / Environment and Plant Protection Institute, CATAS;Hainan Provincial Key Laboratory for Baleful Biology Detection and Monitoring of Tropical Agriculture / Environment and Plant Protection Institute, CATAS |
| |
| Abstract: | Based on the previous study, the Colletotrichum gloeosporioides-specific primers CgInt/ITS4, and the Colletotrichum acutatum-specific primers CaInt2/ITS4 and CAF53/CAR356 were used for taxon-speific amplification to characterize the Colletotrichum species of 138 isolates from Colletotrichum leaf disease lesions on Hevea brasiliensis on the rubber nurseries in China. The results revealed that only 38 isolates belonged to C. acutatum, and 100 isolates to C. gloeosporioides. The rates of C. gloeosporioides on the nurseries in Hainan, Yunnan, Guangdong and Guangxi provinces were 81.82%, 60.0%, 77.27% and 52%, respectively. On the whole, the results indicated that C. gloeosporioides was the dominant species responsible for Colletotrichum leaf disease on H. brasiliensis plants in China. |
| |
| Keywords: | Hevea brasiliensis Colletotrichum gloeosporioides Colletotrichum acutatum Species-specific PCR Molecular identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
