| 甘蔗属热带种与滇蔗茅远缘杂交F_1代花粉母细胞减数分裂及花粉败育研究 |
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| 引用本文： | 林秀琴,陆鑫,刘新龙,毛钧,刘洪博,马丽,徐超华,李旭娟,蔡青.甘蔗属热带种与滇蔗茅远缘杂交F_1代花粉母细胞减数分裂及花粉败育研究[J].热带作物学报,2014,35(9):1776-1783. |
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| 作者姓名： | 林秀琴 陆鑫 刘新龙 毛钧 刘洪博 马丽 徐超华 李旭娟 蔡青 |
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| 作者单位： | 1. 云南省甘蔗遗传改良重点实验室 云南省农业科学院甘蔗研究所 云南开远661699
2. 云南省甘蔗遗传改良重点实验室 云南省农业科学院甘蔗研究所 云南开远661699;云南省农业科学院生物技术与种质资源保存研究所,云南昆明 650223 |
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| 基金项目： | 云南省应用基础研究计划青年项目(No. 2012FD071)；国家现代农业产业技术体系甘蔗产业技术体系开远综合试验站(No. CARS-20-6-13)；农业部作物种质资源保种项目（No. 2014NWB017）。 |
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| 摘 要： | 采用染色体压片法研究甘蔗属热带种与滇蔗茅远缘杂交F1代花粉母细胞减数分裂过程及染色体行为,显微观察结果显示:在终变期观察到单价体、二价体和四价体;分裂中期Ⅰ、后期Ⅰ、中期Ⅱ和后期Ⅱ均出现落后染色体;中期Ⅱ形成"八"字形纺锤丝、后期Ⅱ二分体2个子细胞染色体不同步分离;四分体时期观察到三分体、微核、大小不等的四分体以及多面体等异常细胞;同一小花的3个花药中几个不同时期的分裂相同时存在。进一步应用DAPI荧光染料进行染色观察,结果表明,大多数小孢子在单核时期就发生皱缩变形。该结果说明减数分裂过程异常染色体行为和异常细胞分裂是导致杂交F1代花粉完全败育的主要原因。花粉败育时期为小孢子母细胞时期、四分体时期和单核小孢子时期。
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| 关 键 词： | 甘蔗 滇蔗茅 杂交F1代 花粉败育 减数分裂 |
Meiosis of Pollen Mother Cells and Pollen Abortion in Sugarcane officinarum × Erianthus rockii F1 Hybrids |
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| LIN Xiuqin,LU Xin,LIU Xinlong,MAO Jun,LIU Hongbo,MA Li,XU Chaohu,LI Xujuan and CAI Qing.Meiosis of Pollen Mother Cells and Pollen Abortion in Sugarcane officinarum × Erianthus rockii F1 Hybrids[J].Chinese Journal of Tropical Crops,2014,35(9):1776-1783. |
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| Authors: | LIN Xiuqin LU Xin LIU Xinlong MAO Jun LIU Hongbo MA Li XU Chaohu LI Xujuan and CAI Qing |
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| Institution: | Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane;1 Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences/Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane 2 Biotechnology and Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences |
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| Abstract: | |
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| Keywords: | Sugarcane Erianthus rockii F1 hybrid Pollen abortion Meiosis |
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