不同处理对刺葡萄愈伤组织花青素和原花青素生物合成的影响

以刺葡萄 2 个同质不同型的愈伤组织细胞系为材料，研究不同处理因子对其花青素和原花青素合成能力的影响。试验结果表明，刺葡萄 DLR 细胞系具有高产花青素和原花青素的能力，在培养至 45 d 时，其花青素与原花青素的含量均达到最高值，分别为 113.85 μg/g（FW）和 3 562.95 μg/g（FW）；低温、高温、肉桂酸、壳聚糖、紫外线、黑暗和 KT 等因子，对 DLR 细胞系花青素和原花青素的累积表现出不同的效应；其中，4 ℃低温处理下，DLR 细胞花青素的积累效果最佳，比对照提高了 2.14 倍；壳聚糖处理下，原花青素积累效果最佳，是对照的 2.84 倍。刺葡萄 DLW 细胞系不具有或极低的花青素和原花青素合成能力，处理因子调控对其作用效果不明显。本研究的结论为进一步进行刺葡萄细胞花青素和原花青素合成调控奠定了础。

Effects of Different Treatments on Anthocyanins and Procyanidins Biosynthesis in Spine Grape Callus

Using two homogenous genotype spine grape cell lines with different phenotype as materials, the effects of  different treatment factors on the biosynthesis of anthocyanins and proanthocyanidins were investigated in this experiment. The results showed that DLR cell line had the potential of high-yield anthocyanins and proanthocyanidins. The highest content of anthocyanin and proanthocyanidins, 113.85 μg/g (FW) and 3 562.95 μg/g (FW), respectively, was observed cultured 45 days after. The accumulation effects of anthocyanin and proanthocyanidins in the DLR cells were various under different treatment factors, such as low temperature, high temperature, cinnamic acid, chitosan, ultraviolet, dark, KT (kinetin) and etc. The accumulation effect of anthocyanin in DLR cells was optimal cultured at 4 ℃, which was 2.14 times higher than that of the control. The accumulation effect of proanthocyanidins in DLR cells was optimal under chitosan treatment, which was 2.84 times higher than that of the control. The DLW cell line couldn’t synthesize anthocyanins or proanthocyanidins, and the regulation effect of treatment factors was not obvious. The conclusion of this study laid a foundation for the regulation of anthocyanin and proanthocyanidins biosynthesis in spine grape cells.