| 木薯耐寒相关microRNA的差异表达分析 |
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| 引用本文： | 薄维平,曾长英,宋顺,周玉飞,王文泉.木薯耐寒相关microRNA的差异表达分析[J].热带作物学报,2010,31(8):1260-1265. |
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| 作者姓名： | 薄维平 曾长英 宋顺 周玉飞 王文泉 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,海南海口,571101;海南大学农学院,海南海口,571101
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家973项目"重要热带作物木薯品种改良的基础研究" |
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| 摘 要： | 采用荧光实时定量PCR(quantitative Real Time-PCR,qRT-PCR)技术对低温胁迫处理及对照的两个木薯品种C4和KU50材料进行差异表达分析。结果表明,该6个miRNA在胁迫材料与对照中的表达不同,不同品种及器官中也存在差异表达,且6个miRNA属于受低温诱导与上游调节基因相关的miRNA,这将成为今后研究的主要对象。
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| 关 键 词： | 木薯 耐低温 差异表达 |
Analysis of Differential Expression of Cold-tolerance Related microRNA in Cassava |
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| Bo Weiping,Zeng Changying,Song Shun,Zhou Yufei and Wang Wenquan.Analysis of Differential Expression of Cold-tolerance Related microRNA in Cassava[J].Chinese Journal of Tropical Crops,2010,31(8):1260-1265. |
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| Authors: | Bo Weiping Zeng Changying Song Shun Zhou Yufei and Wang Wenquan |
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| Institution: | 1 Institute of Biotechnology and Bioscience, CATAS 2 College of Agronomy, Hainan University;Institute of Biotechnology and Bioscience, CATAS;1 Institute of Biotechnology and Bioscience, CATAS 2 College of Agronomy, Hainan University;1 Institute of Biotechnology and Bioscience, CATAS 2 College of Agronomy, Hainan University;Institute of Biotechnology and Bioscience, CATAS |
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| Abstract: | qRT-PCR(quantitative Real Time-PCR)strategy was used to analyze the different expression of 6 cold-tolerance related microRNAs in cassava under normal control condition and cold stress. The result indicated that they had differential expression in Cassava leaf and root under different cold treatments. Also, the 6 miRNAs were up-regulate gene related miRNAs induced by low temperature, and they were the target miRNAs in future study. |
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| Keywords: | miRNA qRT-PCR |
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