| 芦笋枯萎病病原鉴定 |
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| 引用本文： | 郑金龙,张世清,高建明,陈河龙,刘巧莲,易克贤.芦笋枯萎病病原鉴定[J].热带作物学报,2010,30(11):33-36. |
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| 作者姓名： | 郑金龙 张世清 高建明 陈河龙 刘巧莲 易克贤 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101;中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101;中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101;中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101;中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101;1 中国热带农业科学院热带生物技术研究所/农业部热带作物生物技术重点开放实验室 海南海口 571101；2 中国热带农业科学院环境与植物保护研究所 海南儋州 571737 |
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| 摘 要： | 对造成福建省漳州市东山县的芦笋枯萎病病原菌进行分子鉴定，以期确定病原菌的属、种名，为防治该病害寻求理论依据。试验对分离纯化的病原菌核糖体DNA的ITS区进行测序，在Genbank中搜索其同源性并构建它们的系统发育树，结果表明：引起芦笋枯萎病的病原菌是镰刀属中的尖孢镰刀菌(Fusarium oxysporum)，且病原菌之间的同源性高达89%，遗传差异不显著。
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| 关 键 词： | 芦笋 枯萎病 rDNA-ITS 鉴定 |
Identification of the Pathogen of Fusarium Wilt of Asparagus |
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| Institution: | Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;1 Institute of Tropical Bioscience and Biotechnology / Ministry of Agriculture Key Laboratory of Tropical Crop Biotechnology, CATAS, Haikou, Hainan 571101;2 Institute of Environment and Plant Protection, CATAS, Danzhou, Hainan 571737, China |
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| Abstract: | Molecular identification was carried out to identify the pathogens and species which caused asparagus Fusarium wilt in Dongshan County, Zhangzhou City, Fujian so as to provide the theoretical basis for prevention and treatment of this disease. The ITS area in the ribosomal DNA was sequenced from the purified pathogens. The homology was searched in gene-bank and the phylogenetic tree was constructed. The results showed that the pathogen which caused asparagus Fusarium wilt was Fusarium oxysporum of the Fusarium genus. And the homology between the pathogens was up to 89%, the genetic difference was not significant. |
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| Keywords: | asparagus Fusarium Wilt rDNA-ITS Identification |
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