猪笼草的组织培养

以猪笼草（Nepenthes mirabilis）腋芽为试材,研究猪笼草的组培快繁。结果表明,外殖体的建立以1/2MS+6-BA 1mg/L+NAA 0.05mg/L液体培养基春季（3～6月）进行培养最佳。愈伤组织（或不定芽）增殖以 MS+6-BA 1mg/L+Ad 10mg/L+NAA 0.1mg/L+100mL/L椰子汁较佳,高浓度（200mL/L）的椰子汁不利于愈伤组织（或不定芽）增殖;继代芽的增殖以MS+6-BA 1.5mg/L+Ad 1～5mg/L+NAA 0.1mg/L+100～200mL/L椰子汁较好:生根培养用1/2MS+6-BA0.1mg/L+NAA1.0mg/L,20d后可长出2～4条根。 

Rapid Propagation of Nepenthes mirabilis by Tissue Culture

An experiment was made with axillary buds of nepenthes mirabilis to investigate the optimum conditions for rapid propagation by tissue culture. The results showed that the liquid 1/2MS medium supplemented with 1mg/L 6-BA and 0.05mg/L NAA was optimal for the production of virus-free materials and that the optimal period was from March to May. The optimal medium for proliferation of callus (or adventitious buds) was MS+6-BA1 mg/L+Ad10 mg/L+NAA 0.1 mg/L+ 100 mL/L coconut milk, but high concentration of coconut milk (200mL/L) had inhibitory effect. The optimal medium for subculture was MS+6-BA1.5 mg/L+Ad 1~5 mg/L+NAA 0.1 mg/L+ 100~200 mL/L coconut milk, and 1/2 MS+6-BA0.1 mg/L+NAA 1.0 mg/L for rooting.