| 高效快速甘蔗转基因方法探索 |
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| 引用本文： | 王文治,杨志坚,杨本鹏,熊国如,蔡文伟,冯翠莲,王俊刚,伍苏然,张树珍.高效快速甘蔗转基因方法探索[J].热带作物学报,2012,33(9):1619-1624. |
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| 作者姓名： | 王文治 杨志坚 杨本鹏 熊国如 蔡文伟 冯翠莲 王俊刚 伍苏然 张树珍 |
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| 作者单位： | 1. 中国热带农业科学院甘蔗研究中心 中国热带农业科学院生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室 海南海口571101
2. 福建农林大学农产品品质研究所,福建福州,350002 |
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| 基金项目： | 现代农业产业技术体系建设专项资金（nycytx-24）；中央级公益科研院所基本科研业务费（ITBB110504）。 |
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| 摘 要： | 甘蔗遗传背景复杂,转基因技术是甘蔗品种遗传改良的重要途径。甘蔗转基因遗传改良主要是通过基因枪法和农杆菌介导法来实现,但目前我国的甘蔗转基因技术,普遍存在转基因效率偏低,获得的转基因株系偏少,难以筛选到目的基因稳定表达、有利用价值的转基因株系等问题。本实验室通过多年的经验积累,建立了一种高效快速的农杆菌介导法的甘蔗转基因方法。转化效率为20%,筛选效率达到90%,转化时间为4个月,操作简单,成本较低。
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| 关 键 词： | 甘蔗 农杆菌介导法 GUS CFP |
A Fast and Efficient Gene Transformation Method for Sugarcane |
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| WANG Wenzhi,YANG Zhijian,YANG Benpeng,XIONG Guoru,CAI Wenwei,FENG Cuilian,WANG Jungang,WU Shuran and ZHANG Shuzhen.A Fast and Efficient Gene Transformation Method for Sugarcane[J].Chinese Journal of Tropical Crops,2012,33(9):1619-1624. |
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| Authors: | WANG Wenzhi YANG Zhijian YANG Benpeng XIONG Guoru CAI Wenwei FENG Cuilian WANG Jungang WU Shuran and ZHANG Shuzhen |
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| Institution: | Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Agricultural Product Quality Institute, Fujian Agricultural and forestry University;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS;Sugarcane Research Center, The Institute of Tropical Bioscience and Biotechnology, Institute of Tropical Bioscience and Biotechnology CATAS |
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| Abstract: | Due to the complexity of genome background in sugarcane, gene trangsformation has been one of the most useful ways for sugarcane genetic improvement. But low efficiency and instablility are the major problems for gene transformation in sugarcane. After years of sugarcane transformation research, a fast and efficient gene transformation system has been developed in our lab. 20% of transformation efficiency, 90% of selection efficiency and only four months of transformation duration make it a low cost transformation approach. |
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