| 胚挽救技术进行无核葡萄及三倍体新种质创制 |
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| 引用本文： | 程琳琳,王跃进,张剑侠,唐冬梅.胚挽救技术进行无核葡萄及三倍体新种质创制[J].热带作物学报,2012,33(9):1584-1589. |
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| 作者姓名： | 程琳琳 王跃进 张剑侠 唐冬梅 |
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| 作者单位： | 1. 西北农林科技大学园艺学院 农业部西北地区园艺作物生物学与种质创制重点实验室 旱区作物逆境生物学国家重点实验室 陕西杨凌712100;中国热带农业科学院橡胶研究所 省部共建国家重点实验室培育基地-海南省热带作物生理学实验室 海南儋州571737
2. 西北农林科技大学园艺学院 农业部西北地区园艺作物生物学与种质创制重点实验室 旱区作物逆境生物学国家重点实验室 陕西杨凌712100 |
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| 基金项目： | 现代农业产业技术体系专项（No. CARS-30-yz-7）。 |
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| 摘 要： | 以二倍体无核葡萄作母本,自交,与二倍体有核葡萄、四倍体有核葡萄杂交,采用胚挽救技术对其自交、杂交胚珠进行培养。对无核×四倍体有核葡萄杂种幼苗进行了流式细胞仪的染色体技术进行倍性鉴定,并进行胚挽救苗的炼苗移栽。共获得幼苗114个单株,初步鉴定三倍体幼苗4株,胚挽救苗成活82株,成活率平均达90.11%。
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| 关 键 词： | 胚挽救技术 无核葡萄 三倍体 |
Development of Novel Seedless and Triploid Grapevine Germplasm by Embryo Rescue Technique |
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| CHENG Linlin,WANG Yuejin,ZHANG Jianxia and TANG Dongmei.Development of Novel Seedless and Triploid Grapevine Germplasm by Embryo Rescue Technique[J].Chinese Journal of Tropical Crops,2012,33(9):1584-1589. |
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| Authors: | CHENG Linlin WANG Yuejin ZHANG Jianxia and TANG Dongmei |
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| Institution: | 1 Key Laboratory of Horticultural Plant Germplasm Resource Utilizition in Northwest China,Ministry of Agriculture, P.R / State Key Laboratory of Crop Stress Biology for Arid Areas / College of Horticulture,Northwest University A & F,Yangling,Shaanxi 712100,China 2 State Key Laboratory Incubation Base for Cultivation and Physiology of Tropical Crops,Ministry of Agriculture,P.R / Rubber Research Institute,CATAS,Danzhou,Hainan 571737,China |
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| Abstract: | Cross combinations were designed, in which seedless grape cultivars were used as the maternal parents. At the same time, seedless grape cultivars, diploid seeded grape cultivars and tetraploid seeded grape cultivars were used as male parents. Ovules obtained from Self-pollination and cross-pollination were cultured by embryo rescue technique. Ploidies of seedless×seeded tetraploid grapes were identified with flow cytometry. Later, cross progenies were transplanted in greenhouse. 114 seedlings were obtained, during which 4 triploid progenies were obtained with flow cytometry primarily. After being transplanted, 82 seedlings survived and the percentage of survival seedlings in greenhouse was 90.11%. |
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| Keywords: | Embryo rescue Seedless grapevine Triploid Chromosome ploidy |
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