| 不同品种木薯叶绿体比较蛋白质组学初步研究 |
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| 引用本文： | 贺庭琪,徐兵强,郭安平,王力敏,王丹,黄启星,仝征,尹奇,王旭初.不同品种木薯叶绿体比较蛋白质组学初步研究[J].热带作物学报,2013,34(6):1090-1097. |
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| 作者姓名： | 贺庭琪 徐兵强 郭安平 王力敏 王丹 黄启星 仝征 尹奇 王旭初 |
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| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院热带生物技术研究所,海南海口571101
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家重点基础研究发展973计划(No. 2010CB126605)；热带生物技术研究所基本科研业务费专项(No. ITBB110101,02)；海南省自然科学基金项目（No. 3100807）。 |
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| 摘 要： | 以“华南8号”木薯(SC8)和“华南124号”木薯(SC124)的叶绿体作为研究材料,采用改进酚抽提法提取蛋白,通过单向SDS-PAGE电泳和双向SDS-PAGE电泳,比较不同木薯品种叶绿体的蛋白表达谱,并对表达的差异蛋白进行MALDI-TOF MS质谱鉴定,获得15个差异蛋白,其中有6个蛋白在SC124木薯叶绿体中表达较高,9个蛋白表达很低.对蛋白进行功能分析,发现差异蛋白主要参与蛋白翻译后修饰、周转、分子伴侣、碳水化合物运输等过程.通过RT-PCR验证了木薯核酮糖1.5-二磷酸羧化酶、ATP合酶β亚基的基因表达情况,结果表明,ATP合酶β亚基基因表达与蛋白质的表达比较一致,而核酮糖1,5-二磷酸羧化酶基因与蛋白质表达变化不一致.
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| 关 键 词： | 木薯 叶绿体 比较蛋白质组学 质谱 RT-PCR |
Comperative Proteomic of Chloroplast From Different Species of Manihot esculenta |
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| HE Tingqi,XU Bingqiang,GUO Anping,WANG Limin,WANG Dan,HUANG Qixing,TONG Zheng,YIN Qi and WANG Xuchu.Comperative Proteomic of Chloroplast From Different Species of Manihot esculenta[J].Chinese Journal of Tropical Crops,2013,34(6):1090-1097. |
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| Authors: | HE Tingqi XU Bingqiang GUO Anping WANG Limin WANG Dan HUANG Qixing TONG Zheng YIN Qi and WANG Xuchu |
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| Institution: | 1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Colloge of Agriculture, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | |
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| Keywords: | Cassava Chloroplast protein Proteomics Mass spectrometry RT-PCR |
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