| 橡胶树HbEBP1蛋白亚细胞定位初步研究 |
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| 引用本文： | 祝建顺,程汉,肖成江,安泽伟,黄华孙.橡胶树HbEBP1蛋白亚细胞定位初步研究[J].热带作物学报,2013,34(5):815-820. |
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| 作者姓名： | 祝建顺 程汉 肖成江 安泽伟 黄华孙 |
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| 作者单位： | 1. 中国热带农业科学院橡胶研究所 国家橡胶树育种中心 海南儋州571737;海南大学农学院 海南海口 570228
2. 中国热带农业科学院橡胶研究所 国家橡胶树育种中心 海南儋州571737 |
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| 基金项目： | 国家天然橡胶产业体系（No. CARS-34）； 中央级公益性科研院所基本科研业务费专项基金项目（No.1630022011002）。 |
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| 摘 要： | 利用融合PCR法构建HbEBP1基因与绿色荧光蛋白基因的融合表达载体,成功转化拟南芥,借助激光共聚焦显微镜检测HbEBP1-EGFP融合蛋白在转基因植株根细胞中的表达及分布.结果显示:在转基因植株根中观察到强烈荧光信号,并分布于细胞质与细胞核中.结果证实了HbEBP1蛋白同时定位于细胞质与细胞核中.
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| 关 键 词： | 橡胶树 融合PCR HbEBP1蛋白 亚细胞定位 |
The Subcellular Localization of Rubber Tree HbEBP1 Protein |
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| ZHU Jianshun,CHENG Han,XIAO Chengjiang,AN Zewei and HUANG Huasun.The Subcellular Localization of Rubber Tree HbEBP1 Protein[J].Chinese Journal of Tropical Crops,2013,34(5):815-820. |
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| Authors: | ZHU Jianshun CHENG Han XIAO Chengjiang AN Zewei and HUANG Huasun |
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| Abstract: | To investigate the subcellular localization of HbEBP1 and find the clues for its function, a HbEBP1-EGFP fusion protein expression vector was constructed by fusion PCR method, and was transformed into Arabidopsis thaliana. The roots of transgentic plant were observed under the confocal microscope for detecting the EGFP distribution. The green fluorescence signal was observed to distribute both in the cytosol and nuclear in transgenic Arabidopsis root cells. The results showed HbEBP1 was localized to both the cytosol and nuclear. |
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| Keywords: | Rubber tree Fusion PCR HbEBP1 protein Subcellular localization |
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