| MADS-box基因酵母双杂交系统的构建及鉴定 |
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| 引用本文： | 刘菊华,徐碧玉,张 静,张建斌,贾彩红,金志强.MADS-box基因酵母双杂交系统的构建及鉴定[J].热带作物学报,2011,32(7):1337-1341. |
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| 作者姓名： | 刘菊华 徐碧玉 张 静 张建斌 贾彩红 金志强 |
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| 作者单位： | 1. 农业部热带作物生物技术重点开放实验室中国热带农业科学院热带生物技术研究所 海南海口571101
2. 农业部热带作物生物技术重点开放实验室中国热带农业科学院热带生物技术研究所 海南海口571101;中国热带农业科学院海口实验站,海南海口 571101 |
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| 基金项目： | 国家自然科学基金项目(No.30960232); 中央级公益性科研院所基本科研业务费专项资金(No.ITBB110202) |
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| 摘 要： | 为研究香蕉中MADS-box基因相互作用的分子机理,筛选该基因表达产物在体内相互作用的靶蛋白,分别构建诱饵质粒载体和香蕉果实采后2 d的cDNA文库,采用共转化法转入酵母细胞中,经过初步筛选鉴定,证明已成功构建了MADS-box基因的酵母双杂交系统。该结果为发现MADS-box基因表达产物在体内相互作用的靶蛋白奠定了基础。
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| 关 键 词： | MADS-box 酵母双杂交 构建 鉴定 |
Construction and Identification of the Yeast Two-hybrid Vector of MADS-box |
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| LIU Juhu,XU Biyu,ZHANG Jing,ZHANG Jianbin,JIA Caihong and JIN Zhiqiang.Construction and Identification of the Yeast Two-hybrid Vector of MADS-box[J].Chinese Journal of Tropical Crops,2011,32(7):1337-1341. |
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| Authors: | LIU Juhu XU Biyu ZHANG Jing ZHANG Jianbin JIA Caihong and JIN Zhiqiang |
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| Institution: | LIU Juhua1,XU Biyu1,ZHANG Jing1,ZHANG Jianbin1,JIA Caihong1,JIN Zhiqiang1,2 1 Key Laboratory of Tropical Crop Biotechnology,Ministry of Agriculture / Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China 2 Haikou Experimental Station,China |
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| Abstract: | To investigate the interacting molecular mechanism of MADS-box gene and screen target proteins in vivo,abaitvector of MADS-box and a cDNA library of the banana fruit two days postharvest were constructed and transfected to yeast cells.The results of identification indicated that the construction of yeast two-hybrid library was successful.This study might lay a foundation for discovering the interacting proteins of the MADS-box in vivo. |
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| Keywords: | MADS-box Yeast two-hybrid Construction Identification |
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