| 芒果核苷二磷酸激酶(NDPK)基因克隆及表达分析 |
| |
| 引用本文： | 罗睿雄,赵志常,黄建峰,党志国,高爱平.芒果核苷二磷酸激酶(NDPK)基因克隆及表达分析[J].热带作物学报,2016,37(11):2183-2190. |
| |
| 作者姓名： | 罗睿雄 赵志常 黄建峰 党志国 高爱平 |
| |
| 作者单位： | 中国热带农业科学院热带作物品种资源研究所 农业部华南作物基因资源与种质创制重点实验室 国家热带果树品种改良中心,海南儋州,571737 |
| |
| 基金项目： | 物种资源保护项目‘芒果种质资源保护’（No. 16RZZY-01）； 中央级公益性科研院所基本科研业务费专项‘芒果种质资源7个重要亲本材料生殖生物学特性研究’（No. 1630032015025）；农业部公益性行业（农业）科研专项‘芒果产业技术研究与示范’（No. 201203092）。 |
| |
| 摘 要： | 采用3′RACE和RT-PCR技术克隆芒果核苷二磷酸激酶基因全长c DNA序列,并用实时荧光定量PCR分析该基因的表达模式。结果表明:Mi NDPK1基因c DNA全长1 019 bp,开放阅读框为447 bp,编码148个氨基酸。Mi NDPK1蛋白定位于细胞质,无信号肽和跨膜区域,具有典型的核苷二磷酸激酶活性结构域和其他磷酸化活性位点;与麻疯树、橡胶树同源性最高为89%,与菠菜同源性最低为82%。Mi NDPK1在芒果各组织中均有表达,以叶片中表达最高,其次是花和果皮;在芒果进入生殖时期的花芽分化期表达量最高,此后在芒果果实发育进程中逐渐降低并趋于平稳。结合病害处理转录组样本中的差异性表达结果,推测芒果Mi NDPK1基因在花芽分化进程、抗病性诱导及免疫应答通路上发挥重要作用。
|
| 关 键 词： | 芒果 核苷二磷酸激酶 基因克隆 生物信息学分析 表达分析 |
Molecular Cloning of Mango NDPK1 Gene and Its Expression Analysis |
| |
| LUO Ruixiong,ZHAO Zhichang,HUANG Jianfeng,DANG Zhiguo and GAO Aiping.Molecular Cloning of Mango NDPK1 Gene and Its Expression Analysis[J].Chinese Journal of Tropical Crops,2016,37(11):2183-2190. |
| |
| Authors: | LUO Ruixiong ZHAO Zhichang HUANG Jianfeng DANG Zhiguo and GAO Aiping |
| |
| Institution: | Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National Cultivar Improvement Center of Tropical Fruit Tree;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National Cultivar Improvement Center of Tropical Fruit Tree;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National Cultivar Improvement Center of Tropical Fruit Tree;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National Cultivar Improvement Center of Tropical Fruit Tree;Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/National Cultivar Improvement Center of Tropical Fruit Tree |
| |
| Abstract: | |
| |
| Keywords: | Mangifera indica Nucleoside diphosphate kinase Molecular cloning Hioinformatics analysis Expression analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《热带作物学报》浏览原始摘要信息 |
| 点击此处可从《热带作物学报》下载免费的PDF全文 |
|
