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低pH和铝胁迫对胡椒根细胞存活和有机酸分泌的影响
引用本文:祖超,杨建峰,李志刚,王灿,鱼欢,邬华松.低pH和铝胁迫对胡椒根细胞存活和有机酸分泌的影响[J].热带作物学报,2016,37(12):2288-2293.
作者姓名:祖超  杨建峰  李志刚  王灿  鱼欢  邬华松
作者单位:1. 中国热带农业科学院香料饮料研究所,海南万宁,571533;2. 农业部香辛饮料作物遗传资源利用重点实验室,海南万宁,571533;3. 海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁,571533
基金项目:海南省自然科学基金项目(No. 20153102)。
摘    要:以胡椒插条苗为研究对象,通过在低pH和正常pH营养液中添加不同浓度铝,研究胡椒根系细胞存活和有机酸分泌对低pH和铝胁迫的响应。结果表明:pH5.0(低pH)条件下,不添加铝时,胡椒根细胞即表现出坏死症状,当添加80μmol/L铝时,胡椒根细胞开始凋亡,200μmol/L铝添加导致胡椒根细胞全部死亡;pH6.0(正常pH)条件下,当添加80μmol/L铝时,胡椒根细胞出现坏死症状,200μmol/L铝添加导致根尖细胞大量凋亡。由此可见,低pH和铝的双重胁迫加速胡椒根细胞凋亡。低浓度铝添加致使胡椒根系分泌有机酸速率增加,柠檬酸为胡椒适应低pH和铝胁迫分泌的主要有机酸;在低pH和正常pH条件下,40μmol/L铝添加均可诱导胡椒根系柠檬酸分泌速度的增加,苹果酸仅在正常pH条件下低浓度铝胁迫时分泌速率增加。因此,低pH条件下,铝胁迫引起胡椒根系分泌有机酸种类减少,分泌速率降低。研究还发现Al3+是对胡椒毒害最严重的铝形态,是pH4.5土壤中的主要铝形态,目前海南省胡椒主要植区有50%的土壤pH低于4.5,所以对于胡椒园土壤的铝毒害应引起足够重视。

关 键 词:胡椒  低pH  铝胁迫  根系  有机酸

Effects of Low pH and Al Stresses on Root Cell Survival and Organic Aicd Exudation in Black Pepper
ZU Chao,YANG Jianfeng,LI Zhigang,WANG Can,YU Huan and WU Huasong.Effects of Low pH and Al Stresses on Root Cell Survival and Organic Aicd Exudation in Black Pepper[J].Chinese Journal of Tropical Crops,2016,37(12):2288-2293.
Authors:ZU Chao  YANG Jianfeng  LI Zhigang  WANG Can  YU Huan and WU Huasong
Institution:Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul;Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul;Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul;Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul;Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul;Institute of Spice and Beverage, Chinese Academy of Tropical Agriculture Science/Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regul
Abstract:
Keywords:Black pepper  Low pH  Al stress  Root  Organic acid
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