| 芥蓝种质资源遗传多样性的SRAP分析 |
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| 引用本文： | 李桂花,陈汉才,张艳,郭凤仪,李向阳,刘振翔,张桂权.芥蓝种质资源遗传多样性的SRAP分析[J].热带作物学报,2011,32(12):2214-2220. |
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| 作者姓名： | 李桂花 陈汉才 张艳 郭凤仪 李向阳 刘振翔 张桂权 |
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| 作者单位： | 1广东省农业科学院蔬菜研究所 4 华南农业大学农学院;广东省农业科学院蔬菜研究所;广东省农业科学院蔬菜研究所;肇庆学院生命科学学院;广州市农业科学院;广州市农业科学院;华南农业大学农学院 |
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| 基金项目： | 广东省农业科学院蔬菜研究所所长基金，广东省科技计划项目粤援建办[2010]41号资助。 |
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| 摘 要： | 调查56份芥蓝种质的植物学性状,并利用SRAP标记分析其遗传多样性。结果表明,从312对引物中筛选出24对引物,共扩增出稳定清晰的条带747条,其中多态性条带147条,多态性位点比例为19.6%。基于SRAP扩增结果 ,应用NTSYSpc2.1构建聚类树状图谱,供试材料间的遗传相似系数的变化范围是0.524～0.884,在相似系数为0.66的水平上,可将56份芥蓝分为6大类。由于芥蓝原产华南地区,其遗传多样性要小于芸薹属其它蔬菜。
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| 关 键 词： | 芥蓝 SRAP 遗传多样性 聚类分析 |
SRAP Analysis on Genetic Diversity of Chinese Kale Germplasm |
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| LI Guihu,CHEN Hancai,ZHANG Yan,GUO Fengyi,LI Xiangyang,LIU Zhenxiang and ZHANG Guiquan.SRAP Analysis on Genetic Diversity of Chinese Kale Germplasm[J].Chinese Journal of Tropical Crops,2011,32(12):2214-2220. |
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| Authors: | LI Guihu CHEN Hancai ZHANG Yan GUO Fengyi LI Xiangyang LIU Zhenxiang and ZHANG Guiquan |
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| Institution: | 1 Vegetable Research Institute, Guangdong Academy of Agricultural Sciences 4 College of Agronomy, South China Agricultural University;Vegetable Research Institute, Guangdong Academy of Agricultural Sciences;Vegetable Research Institute, Guangdong Academy of Agricultural Sciences;Zhaoqing university college of life sciences;Guangzhou Academy of Agricultural Sciences;Guangzhou Academy of Agricultural Sciences;College of Agronomy, South China Agricultural University |
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| Abstract: | The morphological characteristics of 56 Chinese kale accessions were investigated and the DNA polymorphism was analyzed by sequence related amplified polymorphism(SRAP)markers. A total of 747 bands were amplified using 24 primers selected from 312 primers, of which147 were polymorphic, The polymorphic percentage was 19.6%. Among these germplasm the genetic similarity was ranged from 0.524 to 0.884 by software NTSYSpc2.1 based on SRAP results, Cluster analysis showed that 56 Chinese kale germplasm were divided into six groups on genetic similarity of 0.66. Because Chinese kale was from south China, the genetic diversity of Chinese kale was smaller than other Brassica vegetables. |
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