| 多重PCR快速检测甘蔗转基因成分研究 |
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| 引用本文： | 张 卓,许莉萍,陈平华,李林祥,王恒波,高世武,施桂姣,陈忠伟,项 慰,高三基,刘 迪,邰连赛,陈 容,陈如凯.多重PCR快速检测甘蔗转基因成分研究[J].热带作物学报,2014,35(5):897-903. |
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| 作者姓名： | 张 卓 许莉萍 陈平华 李林祥 王恒波 高世武 施桂姣 陈忠伟 项 慰 高三基 刘 迪 邰连赛 陈 容 陈如凯 |
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| 作者单位： | 1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;西南大学;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;1 福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 2 农业部甘蔗及制品质量监督检验测试中心转基因检测室;福建农林大学农业部福建甘蔗生物学与遗传育种重点实验室 |
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| 基金项目： | 国家自然科学基金项目（No. 31070330）；农业转基因生物安全技术检测项目（No. k42130004）。 |
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| 摘 要： | 以单子叶植物常见外源转基因元件Ubiquitin启动子、NOS启动子、NOS终止子、Bar基因和Bt基因序列设计多重PCR引物,通过对PCR扩增体系中退火温度、Premix TaqTM浓度、引物浓度的优化,建立一种快速检测转基因甘蔗成分的多重PCR方法。结果表明:建立的体系一次能有效检测5个参数,其检测的最低DNA质量百分比可达1.0%,并在多个转基因甘蔗品种检测中得到验证。
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| 关 键 词： | 多重PCR 甘蔗 转基因检测 |
Multiplex PCR for Rapid Detection of Components of Genetically Modified Sugarcane |
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| ZHANG Zhuo,XU Liping,CHEN Pinghu,LI Linxiang,WANG Hengbo,GAO Shiwu,SHI Guijiao,CHEN Zhongwei,XIANG Wei,GAO Sanji,LIU Di,TAI Liansai,CHEN Rong and CHEN Rukai.Multiplex PCR for Rapid Detection of Components of Genetically Modified Sugarcane[J].Chinese Journal of Tropical Crops,2014,35(5):897-903. |
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| Authors: | ZHANG Zhuo XU Liping CHEN Pinghu LI Linxiang WANG Hengbo GAO Shiwu SHI Guijiao CHEN Zhongwei XIANG Wei GAO Sanji LIU Di TAI Liansai CHEN Rong and CHEN Rukai |
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| Institution: | 1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;Southwest University;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;1 Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University 2 GMOs LAB of Quality Supervision Inspection & Testing Center for Sugarcane and Derived Products, Ministry of Agriculture;Key Laboratory of Sugarcane Biology and Genetic Breeding, Fujian Agriculture and Forestry University |
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| Abstract: | Primers were designed for multiplex PCR according to the sequences of Ubiquitin promoter, NOS promoter, NOS terminator, Bar and Bt genes, which are in most genetically modified monocotyledons. A rapid and effective multiplex PCR method was established to detect transgenic sugarcane through optimizing the annealing temperatures, concentrations of Premix TaqTM, primers and DNA of transgenic sugarcane in PCR programs. The multiplex PCR method was verified in detection of known transgenic sugarcane lines. The results showed the developed multiplex PCR method worked well with a limit of DNA of transgenic sugarcane down to 1.0%. |
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| Keywords: | Multiplex PCR Sugarcane Transgenic detection |
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