20个云南无性系茶树良种的DNA指纹图谱构建

以20个云南无性系茶树良种为材料,选用7对多态性丰富、品种区分率高、易统计的ISSR引物对茶树良种进行分析。结果显示,7对ISSR引物共扩增出110条带,其中多态带93个,多态条带比例为84.54%,多态信息量平均为0.417,品种相似性系数在0.574~0.854之间,其中引物UBC835,ISSR2不仅多态性丰富,且单个引物就可区分所有品种,是最有效的核心引物;7对核心引物两两组合的效率分析表明,UBC835/UBC811、ISSR2/UBC835和UBC835/ISSR3是高效引物组合,可以完全有效区分所有品种,且品种相似性系数较低;同时使用15个地方品种验证3个高效引物组合,结果表明,UBC835/ISSR2是最佳引物组合,不仅能有效区分所有云南无性系茶树良种,而且能将云南无性系良种与15个地方品种最有效地区分开。使用品种的国家地区代码、育种单位英文缩写、核心引物名称和分子数据组成云南无性系茶树良种的DNA指纹图谱,不仅包含了品种的重要信息,而且其中的分子数据可用作茶树品种的真伪鉴定和遗传关系分析,为茶树品种的知识产权保护提供有效的科学依据。

Establishment of DNA Fingerprints for 20 Improved Clonal Tea Cultivars from Yunnan, China

In this paper,twenty cultivars developed by two breeding institutes in Yunnan Province were analyzed with ISSR marker.Seven core ISSR primers were used for analyzing these tea cultivars.A total of 110 bands were acquired by PAGE with the core primers,93 of which were polymorphic bands with the percentage of polymorphic bands(PPB)84.54%,and the mean value of polymorphism information content(PIC)reached 0.417;the genetic similarity coefficient of the cultivars was 0.574~0.854.UBC835 and ISSR2 with high PIC va...