| 龙眼品种SRAP指纹检索系统的开发及遗传多样性研究 |
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| 引用本文： | 张立杰,陈秀萍,郑姗,谢丽雪,张小艳,李韬.龙眼品种SRAP指纹检索系统的开发及遗传多样性研究[J].热带作物学报,2015,36(2):219-223. |
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| 作者姓名： | 张立杰 陈秀萍 郑姗 谢丽雪 张小艳 李韬 |
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| 作者单位： | 福建省农业科学院果树研究所;福建省农业科学院果树研究所 2 福建省龙眼枇杷育种工程技术研究中心;福建省农业科学院果树研究所;福建省农业科学院果树研究所;福建省农业科学院果树研究所;福建省农业科学院果树研究所 |
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| 基金项目： | 福建省自然科学基金项目(No. 2012J01100，No. 2013J01098)。 |
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| 摘 要： | 应用SRAP技术开发了龙眼指纹检索系统并进行了遗传多样性分析。12对引物组合在16份龙眼及龙荔种质中共扩增出257条DNA带,其中248条为多态带(占96.5%),平均每个引物扩增多态性带20.7条。17份材料间的遗传相似系数范围为0.399~0.871。利用Me6Em7、Me5Em4、Me2Em8等3对引物开发的指纹检索系统,可以区分全部17份种质。根据相似系数进行聚类分析,16个龙眼品种和龙荔分成2大组,龙眼品种间的聚类结果基本反映了供试材料之间的亲缘关系。
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| 关 键 词： | 龙眼 SRAP 指纹检索系统 遗传多样性 |
Development of Fingerprinting Key and Analysis of Genetic Diversity with SRAP Marker for Longan Cultivars |
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| ZHANG Lijie,CHEN Xiuping,ZHENG Shan,XIE Lixue,ZHANG Xiaoyan and LI Tao.Development of Fingerprinting Key and Analysis of Genetic Diversity with SRAP Marker for Longan Cultivars[J].Chinese Journal of Tropical Crops,2015,36(2):219-223. |
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| Authors: | ZHANG Lijie CHEN Xiuping ZHENG Shan XIE Lixue ZHANG Xiaoyan and LI Tao |
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| Institution: | Fruit Research Institute, FAAS;1 Fruit Research Institute, FAAS 2 Fujian Breeding Engineering and Technology Research Center for Longan & Loquat;Fruit Research Institute, FAAS;Fruit Research Institute, FAAS;Fruit Research Institute, FAAS;Fruit Research Institute, FAAS |
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| Abstract: | SRAP was assessed to develope a fingerprinting key and study the genetic diversity on Longan. A total of 257 DNA bands were amplified by 12 selective primers, 248 of which(96.5%)were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 20.7. The genetic similarlity coefficients of 17 materials ranged from 0.399 to 0.871. A fingerprinting key developed by 3 pairs of primers: Me6Em7, Me5Em4 and Me2Em8 could discriminate 17 materials. UPGMA cluster showed that 17 materials were classified into 2 cluster groups: Dimocarpus longana and Dimocarpus confinis, and the result revealed the closed relationships among cultivars tested. |
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| Keywords: | Longan SRAP Fingerprinting key Genetic diversity |
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