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| 金龟子绿僵菌对红棕象甲的室内致病力测定 | |
| 引用本文： | 张晶,覃伟权,阎伟,彭正强.金龟子绿僵菌对红棕象甲的室内致病力测定[J].热带作物学报,2012,33(5):899-905. |
| 作者姓名： | 张晶 覃伟权 阎伟 彭正强 |
| 作者单位： | 1. 中国热带农业科学院椰子研究所,海南文昌 571339;海南大学环境与植物保护学院,海南儋州 571737 2. 中国热带农业科学院椰子研究所,海南文昌,571339 3. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737 |
| 基金项目： | 海南省自然科学基金项目（No. 310102）；中央级公益性科研院所基本科研业务费专项[No. 2011hzs1J013，YWFZX2010-13（N）]；海南大学“211工程”建设项目；中国热带农业科学院院本级基本科研业务费(No. 1630042012005)。 |
| 摘 要： | 在室内用喷雾法测定13株绿僵菌对红棕象甲的致病力。结果表明，菌株致病力强弱与菌株种类有密切关系，菌株的浓度与累计死亡率成线性关系。菌株对红棕象甲的致病力由强到弱的顺序为：Red2＞Red3＞Gre1＞Yx7＞Yx4＞Gre3＞Red1＞Yx1＞Gre2＞Yx6＞Gre4＞Gre5＞Yx3。接种Red2菌株在孢子浓度为1.0×108个/mL水悬浮剂情况下，对4龄红棕象甲幼虫的LT50为5.23 d。用不同浓度的Red2菌株的孢子油悬浮剂涂抹法接种4龄幼虫，发现其LC50为7.32×106个/mL。另外，经试 |
| 关 键 词： | 金龟子绿僵菌 红棕象甲 致病力测定 |
Detection of Pathoqenicity of Meatarhiziums against Rhynchophorus ferrugineus in Laboratory |
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| ZHANG Jing,QIN Weiquan,YAN Wei and PENG Zhengqiang.Detection of Pathoqenicity of Meatarhiziums against Rhynchophorus ferrugineus in Laboratory[J].Chinese Journal of Tropical Crops,2012,33(5):899-905. | |
| Authors: | ZHANG Jing QIN Weiquan YAN Wei and PENG Zhengqiang |
| Institution: | 1 Coconut Research Institute, CATAS 2 Environment and Plant Protection Institute, Hainan University;Coconut Research Institute, CATAS;Coconut Research Institute, CATAS;Environment and Plant Protection Institute, CATAS |
| Abstract: | The pathogenicity of thirteen Metarhiziums sp.isolates against Rhynchophorus ferrugineus was bioassayed in laboratory by spraying.Close relationship was found between the virulence and the isolates.Linear relationship was found between the concentration and the mortalities.The pathogenicity of the isolates against R.ferrugineus is in the order of Red2>Red3>Gre1>Yx7>Yx4>Gre3>Red1>Yx1>Gre2>Yx6>Gre4>Gre5>Yx3.The LD50 of the fourth instar larvae inoculated with isolate Red2 at 1.0×108 spores/mL aqueous suspension was 5.23 d.When inoculated with different levels of oil miscible flowable concentrate(OF)of Red2,the larvae had a LC50 of 7.23×106 spores/mL.Furthermore,the OF was more effective than aqueous suspension for the larvae,the LT50 of OF inoculated with Red2 at 1.0×108 spores/mL is only 3.81 d. |
| Keywords: | Metarhizium anisopliae var Red palm weevil Pathogenicity bioassay |
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