| 荔枝体细胞胚性培养物的DNA提取方法和RAPD分析 |
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| 引用本文： | 黄素华,赖钟雄.荔枝体细胞胚性培养物的DNA提取方法和RAPD分析[J].热带作物学报,2006,27(4):56-59. |
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| 作者姓名： | 黄素华 赖钟雄 |
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| 作者单位： | 1. 福建农林大学亚热带果树研究所,福州,350002;福建省龙岩学院生物科学与工程系,福建,龙岩,364000
2. 福建省龙岩学院生物科学与工程系,福建,龙岩,364000 |
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| 基金项目： | 国家自然科学基金(39870544)，霍英东教育基金会高等学校青年教师基金(71026)等资助项目的部分内容 |
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| 摘 要： | 采用补加PVP的SDS法,提取得到高质量的荔枝(Litchi chinensis Sonn)胚性培养物基因组DNA。对荔枝胚性培养物的RAPD分析结果表明:荔枝胚性和非胚性愈伤组织、玻璃化胚和白色胚、不同染色体数目的细胞系类型之间在DNA分子标记上存在差异,证实荔枝胚性愈伤组织继代及再分化过程中存在遗传物质的变异。
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| 关 键 词： | 荔枝 胚性培养物 体胚发生 DNA提取 RAPD 遗传变异 |
| 收稿时间： | 2006-08-18 |
| 修稿时间： | 2006-10-20 |
Genomic DNA Extraltion of Cultures and RAPD Analysis in the Process of Somatic Embryogenesis in Litchi |
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| Huang Suhua,Lai Zhongxiong.Genomic DNA Extraltion of Cultures and RAPD Analysis in the Process of Somatic Embryogenesis in Litchi[J].Chinese Journal of Tropical Crops,2006,27(4):56-59. |
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| Authors: | Huang Suhua Lai Zhongxiong |
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| Institution: | 1.Institute of Subtropical Fruits, Fujian Agriculture and Forestry University Fuzhou Fujian 350002 China; 2. Department of Biology, Longyan College, Longyan Fujian Fujian 364000 China |
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| Abstract: | High-quality genomic DNA was extracted from litchi embryogenic cultures by the SDS method plus PVP. The RAPD analysis showed that there were differences in DNA molecular markers among the embryogenic and nonembryogenic calli, the vitrified and the white embryoids and the three screened cell lines with different chromosome numbers, which proved that there existed variation in hereditary substances during the process of subcultures and differentiation of embryogenic calli in litchi. |
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| Keywords: | litchi chinensis Sonn embryogenic cultures somatic embryogenesis DNA extraction RAPD genetic variation |
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