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千层金叶片醇提物抗氧化活性的研究
引用本文:吴英详,余雪芳,詹兴堆,李欣欣,杨超,宗伟勋,李永裕.千层金叶片醇提物抗氧化活性的研究[J].热带作物学报,2019,40(7):1367-1372.
作者姓名:吴英详  余雪芳  詹兴堆  李欣欣  杨超  宗伟勋  李永裕
作者单位:1. 清远市农业科技推广服务中心,广东清远 5115182. 福建农林大学园艺学院,福建福州 3500023. 三明市农业市场与经济信息工作站,福建三明 365000
基金项目:清远市科技计划项目(No.2018A013);国家自然科学基金项目(No.31501694);福建省科技计划项目(No.2018N0003);福州市科技计划项目(榕科[2017]347号)
摘    要:为了探究千层金叶片醇提物的抗氧化活性,本文研究了千层金叶片醇提物及其不同极性部位的抗氧化活性和总多酚含量。研究结果表明:千层金叶片醇提物正丁醇相的总多酚含量最高,为(453.75±0.75) mg/g;75%甲醇粗提物及其正丁醇相对ABTS +自由基的清除能力较抗坏血酸(VC)强;各组分还原力强弱顺序为VC>正丁醇相>75%甲醇粗提物>水相>乙酸乙酯相>BHT>石油醚相;正丁醇相和乙酸乙酯相对DPPH自由基的清除能力较醇提物和水相强,石油醚相最弱;千层金叶片醇提物及其不同极性部位清除ABTS +自由基清除能力、DPPH自由基清除能力及还原力均与其总多酚含量呈正相关。除石油醚相外,千层金叶片醇提物及其不同极性部位均有较强的抗氧化活性,可作为一种良好天然抗氧化剂的物质来源。

关 键 词:千层金叶片  醇提物  总多酚  抗氧化活性  
收稿时间:2018-08-03

Antioxidant Activity of Methanol Extract from Melaleuca bracteata Leaves
WU Yingxiang,YU Xuefang,ZHAN Xingdui,LI Xinxin,YANG Chao,ZONG Weixun,LI Yongyu.Antioxidant Activity of Methanol Extract from Melaleuca bracteata Leaves[J].Chinese Journal of Tropical Crops,2019,40(7):1367-1372.
Authors:WU Yingxiang  YU Xuefang  ZHAN Xingdui  LI Xinxin  YANG Chao  ZONG Weixun  LI Yongyu
Institution:1. Qingyuan Agricultural Science and Technology Extension Service Center, Qingyuan, Guangdong 511518, China2. College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China3. Sanming Agricultural Market and Economic Information Workstation, Sanming, Fujian 365000, China
Abstract:In order to explore the antioxidant activity of the methanol extract from the leaves of M. bracteata, the in vitro antioxidant activities and the total phenols of the methanol extract and its different polarity fractions were determined in this study. The n-butyl alcohol fraction showed the highest total phenols content (453.75±0.75) mg/g. The methanol extract and its n-butyl alcohol fraction had better scavenging effects than VC on ABTS + radical. Reducing power followed the order of VC>n-butyl alcohol>methanol extract>water>ethyl acetate>BHT>petroleum ether fractions. The N-butyl alcohol and ethyl acetate fractions had better scavenging effect on DPPH radical than the methanol extract and water fractions, and the petroleum ether fraction had the weakest effect. In addition, positive correlation was existed between the total phenols content and the antioxidant activities of different polarity fractions. In addition to the petroleum ether fraction, methanol extract and its different polarity fractions showed excellent antioxidant activities, which could be used as a good natural antioxidant activity material.
Keywords:Melaleuca bracteata leaves  alcohol extract  total polyphenol  antioxidant activity  
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