枯草芽孢杆菌(Bacillus subtilis)CS27液体发酵条件优化

从提高细胞生物量并降低发酵生产成本的目的出发,本文采用单因素试验和正交试验方法,对已筛选出的枯草芽孢杆菌CS27菌株进行液体发酵培养基和工艺条件优化。得到最佳培养基配方为:1.5%黄豆粉、0.5%糖蜜、0.8%氯化铵、1.0%氯化钠、0.1%柠檬酸钠、0.5%碳酸钙、0.1%七水合硫酸镁;最佳发酵条件为:发酵温度32℃,最佳装液量50 mL/250 mL,最适接种量10%,初始pH7.0。在优化后的培养基发酵条件下细胞生物量可以达到1.75×10~9 cfu/mL,为枯草芽孢杆菌CS27菌株规模化生产及应用提供科学依据。

Optimization of Liquid Fermentation Conditions of Bacillus subtilis CS27

The fermentation medium and culture conditions were optimized by single factor and orthogonal experiments. The optimum fermentation medium was 1.5% soybean powder, 0.5% molasses, 0.8% ammonium chloride, 1.0% NaCl, 0.1% sodium citrate, 0.5% calcium carbonate, 0.1% MgSO₄·7H₂O. The optimum fermentation conditions were fermentation temperature 32 ℃, liquid volume 50 mL / 250 mL, inoculation amount 10%, initial pH value 7.0. The cell biomass could reach 1.75 × 10 ⁹cfu/mL under the optimized fermentation medium, which could provide a scientific basis for the large scale production and application of B. subtilis CS27.